Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20797 | 3' | -51.5 | NC_004689.1 | + | 25456 | 0.66 | 0.933867 |
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Target: 5'- uCCCAC-UGGCcGGAuCGAUcuucGGCGGgCCu -3' miRNA: 3'- -GGGUGcACCGuUUUuGCUA----CCGUC-GG- -5' |
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| 20797 | 3' | -51.5 | NC_004689.1 | + | 2544 | 0.66 | 0.939121 |
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Target: 5'- uCCCAgcgauaUGUGGgGAuuACGGUGGCccucagAGCa -3' miRNA: 3'- -GGGU------GCACCgUUuuUGCUACCG------UCGg -5' |
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| 20797 | 3' | -51.5 | NC_004689.1 | + | 54845 | 0.66 | 0.939121 |
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Target: 5'- aCgGCgGUGGgAAcgGGGCGGUGGUGGCg -3' miRNA: 3'- gGgUG-CACCgUU--UUUGCUACCGUCGg -5' |
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| 20797 | 3' | -51.5 | NC_004689.1 | + | 63745 | 0.66 | 0.9441 |
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Target: 5'- cUCCGCGUGGUccaugucguAGGCGAUGucGUacauGGCCg -3' miRNA: 3'- -GGGUGCACCGuu-------UUUGCUAC--CG----UCGG- -5' |
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| 20797 | 3' | -51.5 | NC_004689.1 | + | 54777 | 0.66 | 0.948346 |
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Target: 5'- aCCCGCcagGGCGuuggcucGGGGCaGAUGGCcgaccagacgGGCCa -3' miRNA: 3'- -GGGUGca-CCGU-------UUUUG-CUACCG----------UCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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