Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20797 | 3' | -51.5 | NC_004689.1 | + | 19312 | 0.66 | 0.928334 |
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Target: 5'- aCgACGgccuUGGCAAGAucgcggaacuGCGGaGGCAGCUu -3' miRNA: 3'- gGgUGC----ACCGUUUU----------UGCUaCCGUCGG- -5' |
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| 20797 | 3' | -51.5 | NC_004689.1 | + | 57373 | 0.66 | 0.928334 |
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Target: 5'- --aGCGUGaGCGAuguuGAACGcguaaccGGCAGCCg -3' miRNA: 3'- gggUGCAC-CGUU----UUUGCua-----CCGUCGG- -5' |
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| 20797 | 3' | -51.5 | NC_004689.1 | + | 51421 | 0.67 | 0.916434 |
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Target: 5'- gCCCaagGCGUaccGGCGAAGAUGGUauucGCGGUCg -3' miRNA: 3'- -GGG---UGCA---CCGUUUUUGCUAc---CGUCGG- -5' |
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| 20797 | 3' | -51.5 | NC_004689.1 | + | 7357 | 0.67 | 0.909416 |
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Target: 5'- cCCCGgcUGUGGUAcauguccGAGGCu-UGGCGGCUg -3' miRNA: 3'- -GGGU--GCACCGU-------UUUUGcuACCGUCGG- -5' |
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| 20797 | 3' | -51.5 | NC_004689.1 | + | 33936 | 1.16 | 0.001209 |
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Target: 5'- aCCCACGUGGCAAAAACGAUGGCAGCCg -3' miRNA: 3'- -GGGUGCACCGUUUUUGCUACCGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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