Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20797 | 5' | -57 | NC_004689.1 | + | 31126 | 0.68 | 0.57435 |
Target: 5'- cGGAGUGCCCCacacGUgcgUCUGCCGUgCGu -3' miRNA: 3'- aCUUCGCGGGGc---CAag-AGACGGUA-GC- -5' |
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20797 | 5' | -57 | NC_004689.1 | + | 459 | 0.69 | 0.522058 |
Target: 5'- -uGAGCGCgUUCGGUgcuucuacacgCUCUGCCAUCu -3' miRNA: 3'- acUUCGCG-GGGCCAa----------GAGACGGUAGc -5' |
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20797 | 5' | -57 | NC_004689.1 | + | 15728 | 0.78 | 0.153697 |
Target: 5'- aGcGGUGCCuuGGgcuugcUCUCUGCCAUCGa -3' miRNA: 3'- aCuUCGCGGggCCa-----AGAGACGGUAGC- -5' |
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20797 | 5' | -57 | NC_004689.1 | + | 33901 | 1.08 | 0.001014 |
Target: 5'- uUGAAGCGCCCCGGUUCUCUGCCAUCGu -3' miRNA: 3'- -ACUUCGCGGGGCCAAGAGACGGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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