Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20798 | 5' | -53.6 | NC_004689.1 | + | 4813 | 0.66 | 0.846778 |
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Target: 5'- cCUUGAUUGcuucacGCUUGaaCGUggUGCGGAa -3' miRNA: 3'- uGAACUAGC------CGAACg-GCGuuACGCCU- -5' |
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| 20798 | 5' | -53.6 | NC_004689.1 | + | 16706 | 0.66 | 0.819866 |
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Target: 5'- --aUGAUC---UUGCCGuCGAUGCGGAa -3' miRNA: 3'- ugaACUAGccgAACGGC-GUUACGCCU- -5' |
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| 20798 | 5' | -53.6 | NC_004689.1 | + | 49237 | 0.66 | 0.819866 |
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Target: 5'- --aUGAUCGGC-UGCUG--GUGCGGc -3' miRNA: 3'- ugaACUAGCCGaACGGCguUACGCCu -5' |
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| 20798 | 5' | -53.6 | NC_004689.1 | + | 25892 | 0.68 | 0.750602 |
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Target: 5'- cGCUUGAUCaGGUUUucGCCGgA--GCGGAa -3' miRNA: 3'- -UGAACUAG-CCGAA--CGGCgUuaCGCCU- -5' |
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| 20798 | 5' | -53.6 | NC_004689.1 | + | 37410 | 0.68 | 0.707013 |
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Target: 5'- cGC-UGGUCGgacuacgcccGCUUGCCGUAGaugcgcuugccgcUGCGGAa -3' miRNA: 3'- -UGaACUAGC----------CGAACGGCGUU-------------ACGCCU- -5' |
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| 20798 | 5' | -53.6 | NC_004689.1 | + | 34686 | 1.09 | 0.001858 |
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Target: 5'- aACUUGAUCGGCUUGCCGCAAUGCGGAu -3' miRNA: 3'- -UGAACUAGCCGAACGGCGUUACGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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