Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20799 | 3' | -49.9 | NC_004689.1 | + | 62498 | 0.67 | 0.925199 |
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Target: 5'- --cGAUGUcauaGCCUUCgGGCACAu- -3' miRNA: 3'- cuaUUACGuug-CGGAAGgCCGUGUuu -5' |
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| 20799 | 3' | -49.9 | NC_004689.1 | + | 26744 | 0.68 | 0.88343 |
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Target: 5'- -----cGCAACuucguuaaccCCUUCCGGCACAGu -3' miRNA: 3'- cuauuaCGUUGc---------GGAAGGCCGUGUUu -5' |
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| 20799 | 3' | -49.9 | NC_004689.1 | + | 12447 | 0.68 | 0.875455 |
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Target: 5'- --cGGUGCAACGCCgUCCGuGC-CGu- -3' miRNA: 3'- cuaUUACGUUGCGGaAGGC-CGuGUuu -5' |
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| 20799 | 3' | -49.9 | NC_004689.1 | + | 11487 | 0.69 | 0.84991 |
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Target: 5'- gGAUGAUGaCAACGCCgacgaUUCCGGuCugGc- -3' miRNA: 3'- -CUAUUAC-GUUGCGG-----AAGGCC-GugUuu -5' |
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| 20799 | 3' | -49.9 | NC_004689.1 | + | 3363 | 0.77 | 0.399426 |
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Target: 5'- -----cGCAACGCCUUuuGGUGCAGAu -3' miRNA: 3'- cuauuaCGUUGCGGAAggCCGUGUUU- -5' |
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| 20799 | 3' | -49.9 | NC_004689.1 | + | 35174 | 1.07 | 0.004814 |
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Target: 5'- cGAUAAUGCAACGCCUUCCGGCACAAAg -3' miRNA: 3'- -CUAUUACGUUGCGGAAGGCCGUGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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