Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20802 | 5' | -58.9 | NC_004689.1 | + | 62089 | 0.67 | 0.481593 |
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Target: 5'- uGCUCGGUGCGCgGcUCuccCGCGUCGAu -3' miRNA: 3'- -CGGGUCGCGCGgU-AGuc-GCGUAGCUu -5' |
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| 20802 | 5' | -58.9 | NC_004689.1 | + | 65566 | 0.67 | 0.461935 |
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Target: 5'- cGUCCAGCGUGgagaaCAUCGGCGUGuagauuUCGAc -3' miRNA: 3'- -CGGGUCGCGCg----GUAGUCGCGU------AGCUu -5' |
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| 20802 | 5' | -58.9 | NC_004689.1 | + | 9535 | 0.67 | 0.461935 |
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Target: 5'- aGCCau-CGuCGCC-UCAGCGUGUCGAAu -3' miRNA: 3'- -CGGgucGC-GCGGuAGUCGCGUAGCUU- -5' |
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| 20802 | 5' | -58.9 | NC_004689.1 | + | 29874 | 0.68 | 0.452266 |
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Target: 5'- aGCCCAGCGUuggcaccgucGCCAcCAGCGUAa---- -3' miRNA: 3'- -CGGGUCGCG----------CGGUaGUCGCGUagcuu -5' |
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| 20802 | 5' | -58.9 | NC_004689.1 | + | 15848 | 0.68 | 0.433266 |
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Target: 5'- gGCCCAGCaGCGCgcggggacaCGUC-GCGCuuGUCGGAc -3' miRNA: 3'- -CGGGUCG-CGCG---------GUAGuCGCG--UAGCUU- -5' |
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| 20802 | 5' | -58.9 | NC_004689.1 | + | 41028 | 0.68 | 0.414739 |
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Target: 5'- uGCCCAGCacaaCGgCAuUCAGCGCGUCc-- -3' miRNA: 3'- -CGGGUCGc---GCgGU-AGUCGCGUAGcuu -5' |
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| 20802 | 5' | -58.9 | NC_004689.1 | + | 46342 | 0.69 | 0.379187 |
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Target: 5'- aGCUCGGCGUGCguUCGGCGaaugaggAUCGGg -3' miRNA: 3'- -CGGGUCGCGCGguAGUCGCg------UAGCUu -5' |
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| 20802 | 5' | -58.9 | NC_004689.1 | + | 38374 | 0.73 | 0.200183 |
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Target: 5'- gGCCUuauuGUGUGCgAUCAGCGCAUCa-- -3' miRNA: 3'- -CGGGu---CGCGCGgUAGUCGCGUAGcuu -5' |
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| 20802 | 5' | -58.9 | NC_004689.1 | + | 11619 | 0.74 | 0.185079 |
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Target: 5'- cGCCCuGUGUGCCGUCAGCGg--CGGu -3' miRNA: 3'- -CGGGuCGCGCGGUAGUCGCguaGCUu -5' |
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| 20802 | 5' | -58.9 | NC_004689.1 | + | 36991 | 1.07 | 0.000655 |
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Target: 5'- aGCCCAGCGCGCCAUCAGCGCAUCGAAc -3' miRNA: 3'- -CGGGUCGCGCGGUAGUCGCGUAGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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