Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20803 | 5' | -53.3 | NC_004689.1 | + | 337 | 0.66 | 0.852685 |
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Target: 5'- gGGCUgggGuCCGAAAaGGUCUGGguugccuUCGGCg -3' miRNA: 3'- -CUGAa--CuGGCUUUgCCGGACC-------AGUCG- -5' |
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| 20803 | 5' | -53.3 | NC_004689.1 | + | 4518 | 0.67 | 0.836289 |
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Target: 5'- cGGCUUGGCCGGAucguACuGCUugUGGUuguaCGGCa -3' miRNA: 3'- -CUGAACUGGCUU----UGcCGG--ACCA----GUCG- -5' |
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| 20803 | 5' | -53.3 | NC_004689.1 | + | 42176 | 0.67 | 0.827346 |
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Target: 5'- -uCUUgGACCGGAuCGGCCUGcGcUGGCg -3' miRNA: 3'- cuGAA-CUGGCUUuGCCGGAC-CaGUCG- -5' |
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| 20803 | 5' | -53.3 | NC_004689.1 | + | 41950 | 0.67 | 0.808857 |
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Target: 5'- gGACUUGGCCGGu---GCCuucgUGGUCGGa -3' miRNA: 3'- -CUGAACUGGCUuugcCGG----ACCAGUCg -5' |
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| 20803 | 5' | -53.3 | NC_004689.1 | + | 28708 | 0.68 | 0.77678 |
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Target: 5'- cGACUgGucucggcaguauccACCGuaucGGCGGCUUGGUUGGCa -3' miRNA: 3'- -CUGAaC--------------UGGCu---UUGCCGGACCAGUCG- -5' |
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| 20803 | 5' | -53.3 | NC_004689.1 | + | 37033 | 0.7 | 0.649485 |
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Target: 5'- cGACUugagccgauugcacUGGCCGAAcgugacCGGCUUGG-CAGCc -3' miRNA: 3'- -CUGA--------------ACUGGCUUu-----GCCGGACCaGUCG- -5' |
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| 20803 | 5' | -53.3 | NC_004689.1 | + | 37251 | 0.7 | 0.641809 |
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Target: 5'- ---gUGACCGuGAcCGGCgcauccgugCUGGUCAGCa -3' miRNA: 3'- cugaACUGGCuUU-GCCG---------GACCAGUCG- -5' |
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| 20803 | 5' | -53.3 | NC_004689.1 | + | 37321 | 1.13 | 0.001141 |
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Target: 5'- cGACUUGACCGAAACGGCCUGGUCAGCg -3' miRNA: 3'- -CUGAACUGGCUUUGCCGGACCAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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