Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20806 | 3' | -49.3 | NC_004689.1 | + | 67850 | 0.67 | 0.955871 |
Target: 5'- gCGCUGcUGAUCCAcguaggaggaaGCGGGCCAUGc-- -3' miRNA: 3'- -GUGGCaACUAGGU-----------UGCUUGGUACuca -5' |
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20806 | 3' | -49.3 | NC_004689.1 | + | 65273 | 0.67 | 0.954592 |
Target: 5'- gGCCGUUGAcuaacgaaaacuucUCCGGCGcuuuACCGUcGAGc -3' miRNA: 3'- gUGGCAACU--------------AGGUUGCu---UGGUA-CUCa -5' |
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20806 | 3' | -49.3 | NC_004689.1 | + | 6066 | 0.69 | 0.906319 |
Target: 5'- cCACCGUUG-UCCAucGCGGacagACCAaGAGa -3' miRNA: 3'- -GUGGCAACuAGGU--UGCU----UGGUaCUCa -5' |
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20806 | 3' | -49.3 | NC_004689.1 | + | 29021 | 0.73 | 0.733417 |
Target: 5'- gCACC--UGAUCCGGCGAACUGUGcAGa -3' miRNA: 3'- -GUGGcaACUAGGUUGCUUGGUAC-UCa -5' |
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20806 | 3' | -49.3 | NC_004689.1 | + | 38032 | 1.07 | 0.007615 |
Target: 5'- uCACCGUUGAUCCAACGAACCAUGAGUc -3' miRNA: 3'- -GUGGCAACUAGGUUGCUUGGUACUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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