Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20809 | 5' | -56.4 | NC_004689.1 | + | 40267 | 0.66 | 0.730057 |
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Target: 5'- cGgCGuUUGCcCUGCGaaUCGGUCGCGUa- -3' miRNA: 3'- -CgGC-GACGuGACGU--AGCCAGCGUAgc -5' |
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| 20809 | 5' | -56.4 | NC_004689.1 | + | 1879 | 0.66 | 0.71969 |
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Target: 5'- gGCCGUUGgGCaGC-UUGGUCGCcUUGc -3' miRNA: 3'- -CGGCGACgUGaCGuAGCCAGCGuAGC- -5' |
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| 20809 | 5' | -56.4 | NC_004689.1 | + | 53723 | 0.66 | 0.718648 |
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Target: 5'- cGCCGUcacugagUGUGCagGCGUUGaUCGCGUCGu -3' miRNA: 3'- -CGGCG-------ACGUGa-CGUAGCcAGCGUAGC- -5' |
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| 20809 | 5' | -56.4 | NC_004689.1 | + | 21303 | 0.68 | 0.620692 |
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Target: 5'- aGCCGUUGCaaccuGCUGCAacuggcucugcaugUCGGUCcaCAUCu -3' miRNA: 3'- -CGGCGACG-----UGACGU--------------AGCCAGc-GUAGc -5' |
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| 20809 | 5' | -56.4 | NC_004689.1 | + | 48559 | 0.69 | 0.549392 |
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Target: 5'- uGCCGUUGUcC-GCuUCGGUCGCGaCGg -3' miRNA: 3'- -CGGCGACGuGaCGuAGCCAGCGUaGC- -5' |
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| 20809 | 5' | -56.4 | NC_004689.1 | + | 35431 | 0.71 | 0.41761 |
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Target: 5'- uCCGCUGCAUUGCGgagCGGcguugccguacggaUCGCGUgCGu -3' miRNA: 3'- cGGCGACGUGACGUa--GCC--------------AGCGUA-GC- -5' |
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| 20809 | 5' | -56.4 | NC_004689.1 | + | 26060 | 0.73 | 0.334536 |
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Target: 5'- uGCCGCUGCuc-GC-UCGGUCGaAUCGa -3' miRNA: 3'- -CGGCGACGugaCGuAGCCAGCgUAGC- -5' |
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| 20809 | 5' | -56.4 | NC_004689.1 | + | 52220 | 0.73 | 0.310663 |
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Target: 5'- cGCUGCuugaccuucuucuUGCGCUccgguucgGCGUCGGUCGgAUCGg -3' miRNA: 3'- -CGGCG-------------ACGUGA--------CGUAGCCAGCgUAGC- -5' |
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| 20809 | 5' | -56.4 | NC_004689.1 | + | 39193 | 1.11 | 0.000787 |
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Target: 5'- uGCCGCUGCACUGCAUCGGUCGCAUCGa -3' miRNA: 3'- -CGGCGACGUGACGUAGCCAGCGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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