Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20816 | 5' | -53.1 | NC_004689.1 | + | 41186 | 0.67 | 0.831903 |
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Target: 5'- uUCCUCGGGGaUGGaaauuUCCAucgagGCgacacgACCa -3' miRNA: 3'- -AGGAGCCCC-AUCg----AGGUuaa--CGa-----UGG- -5' |
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| 20816 | 5' | -53.1 | NC_004689.1 | + | 40580 | 0.67 | 0.804118 |
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Target: 5'- aUCCcgUUGGGG-AGCaacagcgcgccUCCGAUUGCgccACCg -3' miRNA: 3'- -AGG--AGCCCCaUCG-----------AGGUUAACGa--UGG- -5' |
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| 20816 | 5' | -53.1 | NC_004689.1 | + | 68105 | 0.68 | 0.783689 |
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Target: 5'- gUUUCaGGcaacagcGGUAGCUCCGug-GCUACCg -3' miRNA: 3'- aGGAG-CC-------CCAUCGAGGUuaaCGAUGG- -5' |
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| 20816 | 5' | -53.1 | NC_004689.1 | + | 25499 | 0.69 | 0.701495 |
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Target: 5'- cCCUUGGGGUAGC-CCAuggGU--CCg -3' miRNA: 3'- aGGAGCCCCAUCGaGGUuaaCGauGG- -5' |
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| 20816 | 5' | -53.1 | NC_004689.1 | + | 15758 | 0.73 | 0.506308 |
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Target: 5'- uUCUUCGGGGUGGCcUgCAcgUcGcCUGCCa -3' miRNA: 3'- -AGGAGCCCCAUCG-AgGUuaA-C-GAUGG- -5' |
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| 20816 | 5' | -53.1 | NC_004689.1 | + | 49375 | 1.14 | 0.001074 |
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Target: 5'- uUCCUCGGGGUAGCUCCAAUUGCUACCg -3' miRNA: 3'- -AGGAGCCCCAUCGAGGUUAACGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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