Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20818 | 3' | -56.1 | NC_004689.1 | + | 32814 | 0.66 | 0.767293 |
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Target: 5'- uUCGUGACCUUcgAUGGugauguaaaGCGGCUuGAGc -3' miRNA: 3'- -GGUACUGGAG--UACCug-------CGCCGGuCUC- -5' |
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| 20818 | 3' | -56.1 | NC_004689.1 | + | 28345 | 0.66 | 0.747286 |
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Target: 5'- aUCGUG-CCgc-UGGugGCaGCCGGAGu -3' miRNA: 3'- -GGUACuGGaguACCugCGcCGGUCUC- -5' |
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| 20818 | 3' | -56.1 | NC_004689.1 | + | 17176 | 0.67 | 0.684948 |
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Target: 5'- aCCGUGACCa---GGAUcgGCGGgCAGGGa -3' miRNA: 3'- -GGUACUGGaguaCCUG--CGCCgGUCUC- -5' |
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| 20818 | 3' | -56.1 | NC_004689.1 | + | 48113 | 0.69 | 0.588832 |
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Target: 5'- gCGUGGCCg-AUGGAUGaUGGCguGAGg -3' miRNA: 3'- gGUACUGGagUACCUGC-GCCGguCUC- -5' |
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| 20818 | 3' | -56.1 | NC_004689.1 | + | 12353 | 0.7 | 0.533111 |
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Target: 5'- uCCAUGACCUUgcGGAaaacgaggcgcuugUGCGcCCAGAGa -3' miRNA: 3'- -GGUACUGGAGuaCCU--------------GCGCcGGUCUC- -5' |
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| 20818 | 3' | -56.1 | NC_004689.1 | + | 53861 | 1.12 | 0.000796 |
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Target: 5'- aCCAUGACCUCAUGGACGCGGCCAGAGg -3' miRNA: 3'- -GGUACUGGAGUACCUGCGCCGGUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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