Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2082 | 5' | -55.4 | NC_001348.1 | + | 123113 | 0.66 | 0.902107 |
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Target: 5'- gAGGC--GCUGCGAcggaggugcGCCUGGAUGCg-- -3' miRNA: 3'- -UCCGguUGGCGCU---------CGGAUCUAUGagc -5' |
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| 2082 | 5' | -55.4 | NC_001348.1 | + | 106679 | 0.66 | 0.902107 |
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Target: 5'- gAGGC--GCUGCGAcggaggugcGCCUGGAUGCg-- -3' miRNA: 3'- -UCCGguUGGCGCU---------CGGAUCUAUGagc -5' |
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| 2082 | 5' | -55.4 | NC_001348.1 | + | 107034 | 0.66 | 0.874386 |
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Target: 5'- cGGCCGACgGCugagggucggGAGCCUguguggccgGGGUugUUGg -3' miRNA: 3'- uCCGGUUGgCG----------CUCGGA---------UCUAugAGC- -5' |
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| 2082 | 5' | -55.4 | NC_001348.1 | + | 122758 | 0.66 | 0.874386 |
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Target: 5'- cGGCCGACgGCugagggucggGAGCCUguguggccgGGGUugUUGg -3' miRNA: 3'- uCCGGUUGgCG----------CUCGGA---------UCUAugAGC- -5' |
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| 2082 | 5' | -55.4 | NC_001348.1 | + | 123907 | 0.66 | 0.864586 |
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Target: 5'- -aGCC-ACCGCGcagcaaaccgauggGGCCUGGGUGC-CGc -3' miRNA: 3'- ucCGGuUGGCGC--------------UCGGAUCUAUGaGC- -5' |
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| 2082 | 5' | -55.4 | NC_001348.1 | + | 105885 | 0.66 | 0.864586 |
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Target: 5'- -aGCC-ACCGCGcagcaaaccgauggGGCCUGGGUGC-CGc -3' miRNA: 3'- ucCGGuUGGCGC--------------UCGGAUCUAUGaGC- -5' |
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| 2082 | 5' | -55.4 | NC_001348.1 | + | 106209 | 1.09 | 0.002351 |
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Target: 5'- gAGGCCAACCGCGAGCCUAGAUACUCGa -3' miRNA: 3'- -UCCGGUUGGCGCUCGGAUCUAUGAGC- -5' |
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| 2082 | 5' | -55.4 | NC_001348.1 | + | 123583 | 1.09 | 0.002351 |
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Target: 5'- gAGGCCAACCGCGAGCCUAGAUACUCGa -3' miRNA: 3'- -UCCGGUUGGCGCUCGGAUCUAUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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