miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20820 3' -55.9 NC_004689.1 + 30268 0.66 0.73054
Target:  5'- gAACCGUUucCGGCACcagucAGCGccgucGCUGCGAc -3'
miRNA:   3'- -UUGGCAAc-GCCGUGu----UCGC-----CGAUGCU- -5'
20820 3' -55.9 NC_004689.1 + 22893 0.66 0.709386
Target:  5'- -cCUGUUGCuugaGCAauucuucGGCGGCUGCGAu -3'
miRNA:   3'- uuGGCAACGc---CGUgu-----UCGCCGAUGCU- -5'
20820 3' -55.9 NC_004689.1 + 5675 0.66 0.698691
Target:  5'- -cUCGuUUGCGGCGagAAGCGGCU-UGAg -3'
miRNA:   3'- uuGGC-AACGCCGUg-UUCGCCGAuGCU- -5'
20820 3' -55.9 NC_004689.1 + 63090 0.67 0.655383
Target:  5'- uGCCGaUGCGGCAguUGAGCaGUUGCGc -3'
miRNA:   3'- uUGGCaACGCCGU--GUUCGcCGAUGCu -5'
20820 3' -55.9 NC_004689.1 + 21726 0.73 0.314069
Target:  5'- cGGCgGUUGCGGgGCAGGCGuGUUGgGAg -3'
miRNA:   3'- -UUGgCAACGCCgUGUUCGC-CGAUgCU- -5'
20820 3' -55.9 NC_004689.1 + 54531 1.06 0.0016
Target:  5'- uAACCGUUGCGGCACAAGCGGCUACGAu -3'
miRNA:   3'- -UUGGCAACGCCGUGUUCGCCGAUGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.