Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20820 | 3' | -55.9 | NC_004689.1 | + | 63090 | 0.67 | 0.655383 |
Target: 5'- uGCCGaUGCGGCAguUGAGCaGUUGCGc -3' miRNA: 3'- uUGGCaACGCCGU--GUUCGcCGAUGCu -5' |
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20820 | 3' | -55.9 | NC_004689.1 | + | 54531 | 1.06 | 0.0016 |
Target: 5'- uAACCGUUGCGGCACAAGCGGCUACGAu -3' miRNA: 3'- -UUGGCAACGCCGUGUUCGCCGAUGCU- -5' |
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20820 | 3' | -55.9 | NC_004689.1 | + | 30268 | 0.66 | 0.73054 |
Target: 5'- gAACCGUUucCGGCACcagucAGCGccgucGCUGCGAc -3' miRNA: 3'- -UUGGCAAc-GCCGUGu----UCGC-----CGAUGCU- -5' |
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20820 | 3' | -55.9 | NC_004689.1 | + | 22893 | 0.66 | 0.709386 |
Target: 5'- -cCUGUUGCuugaGCAauucuucGGCGGCUGCGAu -3' miRNA: 3'- uuGGCAACGc---CGUgu-----UCGCCGAUGCU- -5' |
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20820 | 3' | -55.9 | NC_004689.1 | + | 21726 | 0.73 | 0.314069 |
Target: 5'- cGGCgGUUGCGGgGCAGGCGuGUUGgGAg -3' miRNA: 3'- -UUGgCAACGCCgUGUUCGC-CGAUgCU- -5' |
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20820 | 3' | -55.9 | NC_004689.1 | + | 5675 | 0.66 | 0.698691 |
Target: 5'- -cUCGuUUGCGGCGagAAGCGGCU-UGAg -3' miRNA: 3'- uuGGC-AACGCCGUg-UUCGCCGAuGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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