Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20821 | 3' | -56.2 | NC_004689.1 | + | 32084 | 0.66 | 0.75073 |
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Target: 5'- cGguGUUGGC-CGACCaGUAGAUcguaCCGUUu -3' miRNA: 3'- -UguCAACCGuGCUGG-CAUCUG----GGCGG- -5' |
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| 20821 | 3' | -56.2 | NC_004689.1 | + | 63987 | 0.67 | 0.688647 |
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Target: 5'- cGCAGUaGGCGCGAaucUCGUcggcacugGGACCCucGUCg -3' miRNA: 3'- -UGUCAaCCGUGCU---GGCA--------UCUGGG--CGG- -5' |
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| 20821 | 3' | -56.2 | NC_004689.1 | + | 11100 | 0.67 | 0.67806 |
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Target: 5'- nCGGagGGC-CGACCG-GGAgCCGCUg -3' miRNA: 3'- uGUCaaCCGuGCUGGCaUCUgGGCGG- -5' |
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| 20821 | 3' | -56.2 | NC_004689.1 | + | 11127 | 0.68 | 0.603339 |
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Target: 5'- -gGGUUGGC-CGggggaGCCGgAGGCuuGCCg -3' miRNA: 3'- ugUCAACCGuGC-----UGGCaUCUGggCGG- -5' |
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| 20821 | 3' | -56.2 | NC_004689.1 | + | 68576 | 0.69 | 0.582067 |
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Target: 5'- aGCAG-UGGgACGcCCuggucgucgGUGGACCCGCa -3' miRNA: 3'- -UGUCaACCgUGCuGG---------CAUCUGGGCGg -5' |
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| 20821 | 3' | -56.2 | NC_004689.1 | + | 30192 | 0.69 | 0.582067 |
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Target: 5'- uCAGUUGGgugAUGACCGUucccAGACCgGCa -3' miRNA: 3'- uGUCAACCg--UGCUGGCA----UCUGGgCGg -5' |
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| 20821 | 3' | -56.2 | NC_004689.1 | + | 32023 | 0.69 | 0.571487 |
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Target: 5'- cGCAGUUGGCuucuCGggcACCGU-GAUUCGCUu -3' miRNA: 3'- -UGUCAACCGu---GC---UGGCAuCUGGGCGG- -5' |
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| 20821 | 3' | -56.2 | NC_004689.1 | + | 11724 | 0.73 | 0.360454 |
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Target: 5'- gGCGGUcGGCuuCGACCGUGuGACCgauguaaaCGCCg -3' miRNA: 3'- -UGUCAaCCGu-GCUGGCAU-CUGG--------GCGG- -5' |
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| 20821 | 3' | -56.2 | NC_004689.1 | + | 54797 | 1.12 | 0.000745 |
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Target: 5'- aACAGUUGGCACGACCGUAGACCCGCCa -3' miRNA: 3'- -UGUCAACCGUGCUGGCAUCUGGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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