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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20825 | 3' | -51.6 | NC_004689.1 | + | 5883 | 0.67 | 0.899078 |
Target: 5'- gCAaUGUGUCuUGGGUGACCuGaGUCu -3' miRNA: 3'- aGUaGCACAGcAUCCACUGGuCaCAG- -5' |
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20825 | 3' | -51.6 | NC_004689.1 | + | 2439 | 0.71 | 0.68251 |
Target: 5'- gUAUCG-GUCGUGuGGUGcCCAGUGg- -3' miRNA: 3'- aGUAGCaCAGCAU-CCACuGGUCACag -5' |
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20825 | 3' | -51.6 | NC_004689.1 | + | 60660 | 1.12 | 0.002182 |
Target: 5'- aUCAUCGUGUCGUAGGUGACCAGUGUCg -3' miRNA: 3'- -AGUAGCACAGCAUCCACUGGUCACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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