Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20826 | 3' | -53.9 | NC_004689.1 | + | 36966 | 0.66 | 0.836289 |
Target: 5'- aACUCGggCGUAUUGGCG-GCAGgcaUCGUg -3' miRNA: 3'- -UGAGUa-GCGUAGCCGCaCGUUa--GGCA- -5' |
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20826 | 3' | -53.9 | NC_004689.1 | + | 53235 | 0.68 | 0.738909 |
Target: 5'- cCUCGUCGuCGUCGGuCGgcucgGCAG-CCGg -3' miRNA: 3'- uGAGUAGC-GUAGCC-GCa----CGUUaGGCa -5' |
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20826 | 3' | -53.9 | NC_004689.1 | + | 37647 | 0.72 | 0.502785 |
Target: 5'- uGCUCAUCGCAUCcaCGUGCcauugcgcucauguuAUCCGUg -3' miRNA: 3'- -UGAGUAGCGUAGccGCACGu--------------UAGGCA- -5' |
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20826 | 3' | -53.9 | NC_004689.1 | + | 61163 | 1.08 | 0.002134 |
Target: 5'- gACUCAUCGCAUCGGCGUGCAAUCCGUa -3' miRNA: 3'- -UGAGUAGCGUAGCCGCACGUUAGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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