Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20830 | 3' | -45.3 | NC_004689.1 | + | 38268 | 0.66 | 0.999589 |
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Target: 5'- gGUUCGAugaAAACcGUUGUGacGUGGcAAUCa -3' miRNA: 3'- gUAAGCU---UUUGcCAACAC--CACC-UUAG- -5' |
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| 20830 | 3' | -45.3 | NC_004689.1 | + | 26698 | 0.66 | 0.999589 |
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Target: 5'- aGUUCGAAAGCG--UGUGucccaGUGGggUUc -3' miRNA: 3'- gUAAGCUUUUGCcaACAC-----CACCuuAG- -5' |
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| 20830 | 3' | -45.3 | NC_004689.1 | + | 18386 | 0.66 | 0.999589 |
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Target: 5'- uCAUagCGAcGAGCGGaacGUccGGUGGAAUCg -3' miRNA: 3'- -GUAa-GCU-UUUGCCaa-CA--CCACCUUAG- -5' |
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| 20830 | 3' | -45.3 | NC_004689.1 | + | 69622 | 0.66 | 0.999337 |
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Target: 5'- --aUCGGcacACGGUUGaUGGUGGcGUUc -3' miRNA: 3'- guaAGCUuu-UGCCAAC-ACCACCuUAG- -5' |
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| 20830 | 3' | -45.3 | NC_004689.1 | + | 47536 | 0.66 | 0.998868 |
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Target: 5'- gGUUCGGAAACGGUUcgcagcgccGGGAUCg -3' miRNA: 3'- gUAAGCUUUUGCCAAcacca----CCUUAG- -5' |
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| 20830 | 3' | -45.3 | NC_004689.1 | + | 58893 | 0.7 | 0.984125 |
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Target: 5'- gAUUCGggGAUGGUcG-GGccGGGAUCg -3' miRNA: 3'- gUAAGCuuUUGCCAaCaCCa-CCUUAG- -5' |
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| 20830 | 3' | -45.3 | NC_004689.1 | + | 43287 | 0.71 | 0.970908 |
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Target: 5'- ---cCGAGAGCGGUUGU-GUGGGc-- -3' miRNA: 3'- guaaGCUUUUGCCAACAcCACCUuag -5' |
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| 20830 | 3' | -45.3 | NC_004689.1 | + | 54839 | 0.71 | 0.963869 |
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Target: 5'- ---gUGGGAACGGggcgGUGGUGGcguGAUCg -3' miRNA: 3'- guaaGCUUUUGCCaa--CACCACC---UUAG- -5' |
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| 20830 | 3' | -45.3 | NC_004689.1 | + | 64412 | 1.13 | 0.008953 |
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Target: 5'- aCAUUCGAAAACGGUUGUGGUGGAAUCa -3' miRNA: 3'- -GUAAGCUUUUGCCAACACCACCUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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