Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20830 | 5' | -56.7 | NC_004689.1 | + | 57710 | 0.68 | 0.590839 |
Target: 5'- uGggGaugUCCUGCCACGGCagguugucgcgcCAGUCCa- -3' miRNA: 3'- -CuuCaa-GGGAUGGUGCCG------------GUCAGGga -5' |
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20830 | 5' | -56.7 | NC_004689.1 | + | 23019 | 0.7 | 0.447199 |
Target: 5'- uAGGUUCCCUucgGCaCACGGCUcagcaAGUCCUc -3' miRNA: 3'- cUUCAAGGGA---UG-GUGCCGG-----UCAGGGa -5' |
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20830 | 5' | -56.7 | NC_004689.1 | + | 68743 | 0.71 | 0.373394 |
Target: 5'- cGAG-UCCCUugacguaACgCACGGCUGGUCCCa -3' miRNA: 3'- cUUCaAGGGA-------UG-GUGCCGGUCAGGGa -5' |
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20830 | 5' | -56.7 | NC_004689.1 | + | 25522 | 0.74 | 0.254165 |
Target: 5'- uGAAGUUCUCUaGCCACGGCUcG-CCCUu -3' miRNA: 3'- -CUUCAAGGGA-UGGUGCCGGuCaGGGA- -5' |
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20830 | 5' | -56.7 | NC_004689.1 | + | 64373 | 0.96 | 0.007927 |
Target: 5'- cGAAGUUCCUcACCACGGCCAGUCCCUc -3' miRNA: 3'- -CUUCAAGGGaUGGUGCCGGUCAGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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