Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20832 | 5' | -53 | NC_004689.1 | + | 19830 | 0.66 | 0.879737 |
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Target: 5'- gCUGCAACGCauuuuCGCCGCuucGGUcuGCGAa -3' miRNA: 3'- -GACGUUGUGcuu--GCGGCG---UCA--CGUUg -5' |
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| 20832 | 5' | -53 | NC_004689.1 | + | 68919 | 0.66 | 0.855518 |
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Target: 5'- uCUGCugUGCGAACGCaCGCAuagcauugGUGUAGu -3' miRNA: 3'- -GACGuuGUGCUUGCG-GCGU--------CACGUUg -5' |
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| 20832 | 5' | -53 | NC_004689.1 | + | 8931 | 0.66 | 0.855518 |
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Target: 5'- -cGCAGCACuuuCGCCuGCGGUGUc-- -3' miRNA: 3'- gaCGUUGUGcuuGCGG-CGUCACGuug -5' |
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| 20832 | 5' | -53 | NC_004689.1 | + | 52428 | 0.67 | 0.829159 |
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Target: 5'- -aGCcuGCGCGggUGCCuuuuucacaGcCGGUGCAGCu -3' miRNA: 3'- gaCGu-UGUGCuuGCGG---------C-GUCACGUUG- -5' |
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| 20832 | 5' | -53 | NC_004689.1 | + | 41723 | 0.67 | 0.819938 |
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Target: 5'- aUGguGCugGAACG-UGUuGUGCAACu -3' miRNA: 3'- gACguUGugCUUGCgGCGuCACGUUG- -5' |
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| 20832 | 5' | -53 | NC_004689.1 | + | 1128 | 0.67 | 0.819938 |
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Target: 5'- uCUGCGAgcCACGua-GCCGCuuUGUAACg -3' miRNA: 3'- -GACGUU--GUGCuugCGGCGucACGUUG- -5' |
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| 20832 | 5' | -53 | NC_004689.1 | + | 67665 | 0.67 | 0.788137 |
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Target: 5'- -aGcCAGcCACGAAUGUCGUcaggugaggggaacGGUGCAGCg -3' miRNA: 3'- gaC-GUU-GUGCUUGCGGCG--------------UCACGUUG- -5' |
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| 20832 | 5' | -53 | NC_004689.1 | + | 41771 | 0.68 | 0.760762 |
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Target: 5'- uUGCAGC-CGGuUGCCGCccAGUGCcuuGACg -3' miRNA: 3'- gACGUUGuGCUuGCGGCG--UCACG---UUG- -5' |
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| 20832 | 5' | -53 | NC_004689.1 | + | 13443 | 0.7 | 0.663741 |
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Target: 5'- aCUGCAACGCGcGACcaguGUCGUAGUcCAGCg -3' miRNA: 3'- -GACGUUGUGC-UUG----CGGCGUCAcGUUG- -5' |
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| 20832 | 5' | -53 | NC_004689.1 | + | 66039 | 1.09 | 0.001859 |
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Target: 5'- aCUGCAACACGAACGCCGCAGUGCAACc -3' miRNA: 3'- -GACGUUGUGCUUGCGGCGUCACGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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