Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20835 | 3' | -59.2 | NC_004689.1 | + | 31440 | 0.67 | 0.495342 |
Target: 5'- cGGGCAACCCGGC--ACUCCUGa--- -3' miRNA: 3'- uUCCGUUGGGCUGggUGAGGGCagau -5' |
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20835 | 3' | -59.2 | NC_004689.1 | + | 32839 | 0.67 | 0.455936 |
Target: 5'- -cGGCAgGCCCGACCCACa--UGUCg- -3' miRNA: 3'- uuCCGU-UGGGCUGGGUGaggGCAGau -5' |
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20835 | 3' | -59.2 | NC_004689.1 | + | 56084 | 0.69 | 0.365507 |
Target: 5'- cGAGGCAGCagCCGACCCACgugcagggCCaaCGUCg- -3' miRNA: 3'- -UUCCGUUG--GGCUGGGUGa-------GG--GCAGau -5' |
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20835 | 3' | -59.2 | NC_004689.1 | + | 6831 | 0.7 | 0.332966 |
Target: 5'- --aGCGGCCUGGCCCACUCCgGcagaagcgaUCUGa -3' miRNA: 3'- uucCGUUGGGCUGGGUGAGGgC---------AGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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