Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20835 | 5' | -61.7 | NC_004689.1 | + | 28225 | 0.66 | 0.458581 |
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Target: 5'- aCCGAAUugccGGUGGgAGaaaCGAGGCUGg -3' miRNA: 3'- aGGCUUGc---CCACCgUCg--GCUCCGGCg -5' |
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| 20835 | 5' | -61.7 | NC_004689.1 | + | 50517 | 0.67 | 0.418605 |
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Target: 5'- aCCGGcucacgccagaacaGCGGGgagGGCuGCCGAGacgaaauGUCGCc -3' miRNA: 3'- aGGCU--------------UGCCCa--CCGuCGGCUC-------CGGCG- -5' |
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| 20835 | 5' | -61.7 | NC_004689.1 | + | 1890 | 0.7 | 0.262369 |
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Target: 5'- aCCGAcacGCGGGccguugGGCAGCUu-GGUCGCc -3' miRNA: 3'- aGGCU---UGCCCa-----CCGUCGGcuCCGGCG- -5' |
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| 20835 | 5' | -61.7 | NC_004689.1 | + | 47237 | 0.7 | 0.250021 |
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Target: 5'- gUCUGAcucgGCGGG-GGCAGUCGcGGaUCGCg -3' miRNA: 3'- -AGGCU----UGCCCaCCGUCGGCuCC-GGCG- -5' |
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| 20835 | 5' | -61.7 | NC_004689.1 | + | 35670 | 0.71 | 0.215843 |
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Target: 5'- cCCGAugGcaacccaccuucGGUGGUcuggucGCCGGGGCUGCu -3' miRNA: 3'- aGGCUugC------------CCACCGu-----CGGCUCCGGCG- -5' |
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| 20835 | 5' | -61.7 | NC_004689.1 | + | 10540 | 0.76 | 0.109771 |
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Target: 5'- gCCGGAgGGGUGGCcguuGCCGGGGgagugggagacgaaCCGCc -3' miRNA: 3'- aGGCUUgCCCACCGu---CGGCUCC--------------GGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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