Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20837 | 3' | -59.8 | NC_004689.1 | + | 52096 | 0.66 | 0.534583 |
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Target: 5'- ----cGGUGGGcUUGUUGCCACcaaUCGCa -3' miRNA: 3'- cgcgaCCGCCCaGACAGCGGUG---AGCG- -5' |
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| 20837 | 3' | -59.8 | NC_004689.1 | + | 28839 | 0.67 | 0.485034 |
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Target: 5'- cCGUUGGCGGGUCgccUGCUGCggGCc -3' miRNA: 3'- cGCGACCGCCCAGacaGCGGUGagCG- -5' |
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| 20837 | 3' | -59.8 | NC_004689.1 | + | 25035 | 0.69 | 0.376324 |
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Target: 5'- cGCGCcagugugagUGGCGGGUUggcguUGUUGaCCACaCGCc -3' miRNA: 3'- -CGCG---------ACCGCCCAG-----ACAGC-GGUGaGCG- -5' |
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| 20837 | 3' | -59.8 | NC_004689.1 | + | 11081 | 0.7 | 0.336132 |
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Target: 5'- cCGCUGGCgGGGUCUGcUGCUGCggGUu -3' miRNA: 3'- cGCGACCG-CCCAGACaGCGGUGagCG- -5' |
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| 20837 | 3' | -59.8 | NC_004689.1 | + | 45037 | 0.7 | 0.32848 |
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Target: 5'- -gGuCUGGCGGGaUUGUCGCa--UCGCa -3' miRNA: 3'- cgC-GACCGCCCaGACAGCGgugAGCG- -5' |
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| 20837 | 3' | -59.8 | NC_004689.1 | + | 42158 | 0.71 | 0.299165 |
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Target: 5'- uGCGCUGGCGGGgaugCgaucagagCGCCGacagCGCc -3' miRNA: 3'- -CGCGACCGCCCa---Gaca-----GCGGUga--GCG- -5' |
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| 20837 | 3' | -59.8 | NC_004689.1 | + | 39852 | 0.78 | 0.094384 |
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Target: 5'- cGCGCUGGCGGGUaucggCGCUgaACUCGUu -3' miRNA: 3'- -CGCGACCGCCCAgaca-GCGG--UGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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