Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20837 | 5' | -52.1 | NC_004689.1 | + | 22341 | 0.66 | 0.907014 |
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Target: 5'- cGGCGA----ACUgACCGGCCAAUc- -3' miRNA: 3'- uUCGCUaaguUGAgUGGCCGGUUGuc -5' |
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| 20837 | 5' | -52.1 | NC_004689.1 | + | 35236 | 0.66 | 0.869867 |
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Target: 5'- -uGCGGgaaugccUAGCUCugCGGCCAauuggauuGCAGg -3' miRNA: 3'- uuCGCUaa-----GUUGAGugGCCGGU--------UGUC- -5' |
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| 20837 | 5' | -52.1 | NC_004689.1 | + | 40809 | 0.67 | 0.853168 |
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Target: 5'- cAGGCG-UUCcuugacgaGGCUCACCGGCaGAUAGc -3' miRNA: 3'- -UUCGCuAAG--------UUGAGUGGCCGgUUGUC- -5' |
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| 20837 | 5' | -52.1 | NC_004689.1 | + | 23367 | 0.67 | 0.835507 |
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Target: 5'- gAAGCGAUUCcaGACcaaUCACCaGCCGGuCGGu -3' miRNA: 3'- -UUCGCUAAG--UUG---AGUGGcCGGUU-GUC- -5' |
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| 20837 | 5' | -52.1 | NC_004689.1 | + | 13334 | 0.67 | 0.835507 |
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Target: 5'- -cGCGGacuccggUGGCUCACCGGCCuguucgugcGGCAGa -3' miRNA: 3'- uuCGCUaa-----GUUGAGUGGCCGG---------UUGUC- -5' |
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| 20837 | 5' | -52.1 | NC_004689.1 | + | 22247 | 0.71 | 0.601302 |
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Target: 5'- gGAGCGGUcCAcguaguuGCg-ACCGGCCAGCGGa -3' miRNA: 3'- -UUCGCUAaGU-------UGagUGGCCGGUUGUC- -5' |
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| 20837 | 5' | -52.1 | NC_004689.1 | + | 53048 | 0.75 | 0.403422 |
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Target: 5'- cGGCGAUUCGACggccCAgCGGCCAaucGCGGu -3' miRNA: 3'- uUCGCUAAGUUGa---GUgGCCGGU---UGUC- -5' |
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| 20837 | 5' | -52.1 | NC_004689.1 | + | 53981 | 0.78 | 0.293477 |
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Target: 5'- uGGGCGAcUUCGcuguACUCACCGGCC-ACGGu -3' miRNA: 3'- -UUCGCU-AAGU----UGAGUGGCCGGuUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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