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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20838 | 3' | -56.9 | NC_004689.1 | + | 44197 | 0.67 | 0.631581 |
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Target: 5'- aGCGGCCaugauUUUGCGAGCUUCuaGUGUGGa -3' miRNA: 3'- -CGUUGG-----AGACGCUUGAGGggCGCGUC- -5' |
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| 20838 | 3' | -56.9 | NC_004689.1 | + | 48866 | 0.68 | 0.567632 |
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Target: 5'- uGCGGCCaUCcGCuGAGCcugCUCCGCGCAc -3' miRNA: 3'- -CGUUGG-AGaCG-CUUGa--GGGGCGCGUc -5' |
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| 20838 | 3' | -56.9 | NC_004689.1 | + | 58434 | 0.69 | 0.524851 |
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Target: 5'- cCGACUgugcCUGCGGGCgaagcuuguugaaUCCCUGCGCAa -3' miRNA: 3'- cGUUGGa---GACGCUUG-------------AGGGGCGCGUc -5' |
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| 20838 | 3' | -56.9 | NC_004689.1 | + | 64882 | 0.69 | 0.48534 |
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Target: 5'- cGCAucCCUCUGCGGugACcugUCCCuUGCGCAc -3' miRNA: 3'- -CGUu-GGAGACGCU--UG---AGGG-GCGCGUc -5' |
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| 20838 | 3' | -56.9 | NC_004689.1 | + | 68471 | 0.71 | 0.391315 |
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Target: 5'- cGCAGuCCguugauucggCUGCGGAaaacUUCUCCGCGCAGu -3' miRNA: 3'- -CGUU-GGa---------GACGCUU----GAGGGGCGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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