Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2084 | 3' | -59.7 | NC_001348.1 | + | 90798 | 0.67 | 0.598011 |
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Target: 5'- ---aACGCAg--CCGAGGAcGCCGCCCu -3' miRNA: 3'- uuugUGCGUgggGGUUCCU-CGGCGGG- -5' |
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| 2084 | 3' | -59.7 | NC_001348.1 | + | 124705 | 0.67 | 0.618377 |
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Target: 5'- gGGACucCGuCACCCCCGacucugcgGGGGGCuccuccccccgCGCCCu -3' miRNA: 3'- -UUUGu-GC-GUGGGGGU--------UCCUCG-----------GCGGG- -5' |
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| 2084 | 3' | -59.7 | NC_001348.1 | + | 106894 | 0.66 | 0.659169 |
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Target: 5'- ---gGCGUAuUCCCCGGGGGGCCcugcauacCCCg -3' miRNA: 3'- uuugUGCGU-GGGGGUUCCUCGGc-------GGG- -5' |
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| 2084 | 3' | -59.7 | NC_001348.1 | + | 113737 | 0.66 | 0.669334 |
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Target: 5'- gAAACACGCugCUCCAA--AGUCuCCCa -3' miRNA: 3'- -UUUGUGCGugGGGGUUccUCGGcGGG- -5' |
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| 2084 | 3' | -59.7 | NC_001348.1 | + | 82884 | 0.66 | 0.679469 |
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Target: 5'- -cACuCGCACCCCCAcggauuagaucgAGGGuCCGUUCu -3' miRNA: 3'- uuUGuGCGUGGGGGU------------UCCUcGGCGGG- -5' |
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| 2084 | 3' | -59.7 | NC_001348.1 | + | 109129 | 0.66 | 0.699613 |
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Target: 5'- ---aGCGCucuACaCCCCAAcgcgcGGGGUCGCCUg -3' miRNA: 3'- uuugUGCG---UG-GGGGUU-----CCUCGGCGGG- -5' |
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| 2084 | 3' | -59.7 | NC_001348.1 | + | 123031 | 0.74 | 0.278573 |
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Target: 5'- gGAAUACGCcgaaauCCCCCGuuuGGGGCCgGUCCg -3' miRNA: 3'- -UUUGUGCGu-----GGGGGUu--CCUCGG-CGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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