miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20841 3' -64.1 NC_004689.1 + 59264 0.66 0.404328
Target:  5'- cGGuCUCCCCUcauggacGCCGGuCCCUGcuCGCGGc -3'
miRNA:   3'- -CC-GAGGGGGc------CGGUU-GGGGCu-GCGCC- -5'
20841 3' -64.1 NC_004689.1 + 31670 0.66 0.379569
Target:  5'- cGUUCCcaguCCCGGCCAcgaACCguuuCCGGCGUGc -3'
miRNA:   3'- cCGAGG----GGGCCGGU---UGG----GGCUGCGCc -5'
20841 3' -64.1 NC_004689.1 + 12753 0.66 0.379569
Target:  5'- --gUCUCCCGGCUgagugccaagcaGACCCuCGGCGuUGGg -3'
miRNA:   3'- ccgAGGGGGCCGG------------UUGGG-GCUGC-GCC- -5'
20841 3' -64.1 NC_004689.1 + 65530 0.66 0.371545
Target:  5'- cGGCggugUCUCCGGCCu-CCaCGuCGCGGc -3'
miRNA:   3'- -CCGa---GGGGGCCGGuuGGgGCuGCGCC- -5'
20841 3' -64.1 NC_004689.1 + 48991 0.67 0.333187
Target:  5'- cGCUCCgacugaUCCGGCUcgacGGCCUCGgacuGCGCGGc -3'
miRNA:   3'- cCGAGG------GGGCCGG----UUGGGGC----UGCGCC- -5'
20841 3' -64.1 NC_004689.1 + 48754 0.67 0.325873
Target:  5'- gGGCaucgCgCUCGGCCuucuGCUCCGGCGUGu -3'
miRNA:   3'- -CCGa---GgGGGCCGGu---UGGGGCUGCGCc -5'
20841 3' -64.1 NC_004689.1 + 52476 0.68 0.304649
Target:  5'- cGGCUCCggcUCCGGCUucGACUcuaccgcuuucaCCGGCGCuGGa -3'
miRNA:   3'- -CCGAGG---GGGCCGG--UUGG------------GGCUGCG-CC- -5'
20841 3' -64.1 NC_004689.1 + 48900 0.7 0.213869
Target:  5'- gGGCUUcggCCUCGGCCuguGCCUCGGCggcacguGCGGc -3'
miRNA:   3'- -CCGAG---GGGGCCGGu--UGGGGCUG-------CGCC- -5'
20841 3' -64.1 NC_004689.1 + 36888 0.76 0.086379
Target:  5'- uGGcCUgCCCCGGUC-GCCCC-ACGCGGg -3'
miRNA:   3'- -CC-GAgGGGGCCGGuUGGGGcUGCGCC- -5'
20841 3' -64.1 NC_004689.1 + 25911 0.77 0.074607
Target:  5'- cGGCUUCCUCGGCCAACUCCGcuugaucagguuuucGCcggaGCGGa -3'
miRNA:   3'- -CCGAGGGGGCCGGUUGGGGC---------------UG----CGCC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.