Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20843 | 5' | -48.8 | NC_004689.1 | + | 18616 | 0.67 | 0.982464 |
Target: 5'- --cCGUCAGCaUGGACaGGAuGGACGc -3' miRNA: 3'- cuaGCAGUUGcACUUGcUCUuCCUGC- -5' |
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20843 | 5' | -48.8 | NC_004689.1 | + | 52291 | 0.67 | 0.975041 |
Target: 5'- --cCGUCuuggGCGUGAGCGAGAccGuGCGg -3' miRNA: 3'- cuaGCAGu---UGCACUUGCUCUucC-UGC- -5' |
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20843 | 5' | -48.8 | NC_004689.1 | + | 67532 | 0.69 | 0.949316 |
Target: 5'- uGUCG-CGAaauggugagaGUGAGCGAGggGGAUu -3' miRNA: 3'- cUAGCaGUUg---------CACUUGCUCuuCCUGc -5' |
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20843 | 5' | -48.8 | NC_004689.1 | + | 64928 | 0.72 | 0.848745 |
Target: 5'- gGAUCGUCGGCGUcGAACaGuAGAccgggccacucGGGACGc -3' miRNA: 3'- -CUAGCAGUUGCA-CUUG-C-UCU-----------UCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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