Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20846 | 3' | -59.7 | NC_004689.1 | + | 30151 | 0.66 | 0.565044 |
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Target: 5'- -uUGGCuAUGGcaACGGCGUUcaacagcagaGCgACCGCCc -3' miRNA: 3'- uuGCCG-UGCC--UGCCGCAA----------CG-UGGCGG- -5' |
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| 20846 | 3' | -59.7 | NC_004689.1 | + | 53811 | 0.66 | 0.553686 |
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Target: 5'- aAGCGGaugaACGGAgGGCcgcucuggggauuGUUGC-CCGCa -3' miRNA: 3'- -UUGCCg---UGCCUgCCG-------------CAACGuGGCGg -5' |
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| 20846 | 3' | -59.7 | NC_004689.1 | + | 7695 | 0.66 | 0.524095 |
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Target: 5'- -uUGGUGCGGugGGUGUacaucgaacccUGCACgauccgUGCCu -3' miRNA: 3'- uuGCCGUGCCugCCGCA-----------ACGUG------GCGG- -5' |
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| 20846 | 3' | -59.7 | NC_004689.1 | + | 26532 | 0.67 | 0.484331 |
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Target: 5'- uGACGaauGCACGcGACGaGCGaagucgUGCGCCGUUg -3' miRNA: 3'- -UUGC---CGUGC-CUGC-CGCa-----ACGUGGCGG- -5' |
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| 20846 | 3' | -59.7 | NC_004689.1 | + | 50187 | 0.67 | 0.464039 |
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Target: 5'- --aGGUACGGAgugccgaUGGCGaUGUagcuGCCGCCc -3' miRNA: 3'- uugCCGUGCCU-------GCCGCaACG----UGGCGG- -5' |
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| 20846 | 3' | -59.7 | NC_004689.1 | + | 40678 | 0.68 | 0.455479 |
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Target: 5'- cGGCGGCGagcauuuCGGCGUUGauCAUCGCCu -3' miRNA: 3'- -UUGCCGUgccu---GCCGCAAC--GUGGCGG- -5' |
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| 20846 | 3' | -59.7 | NC_004689.1 | + | 66061 | 0.69 | 0.366622 |
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Target: 5'- --gGGCACGGAUcGCGguuggUGUACUGCa -3' miRNA: 3'- uugCCGUGCCUGcCGCa----ACGUGGCGg -5' |
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| 20846 | 3' | -59.7 | NC_004689.1 | + | 10592 | 0.73 | 0.200245 |
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Target: 5'- aGACGuuGCGGGCGGUGUUGCagcgggauuggGCUGCUg -3' miRNA: 3'- -UUGCcgUGCCUGCCGCAACG-----------UGGCGG- -5' |
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| 20846 | 3' | -59.7 | NC_004689.1 | + | 41855 | 0.74 | 0.185419 |
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Target: 5'- cGCGGCACGGuCGGCcUUGCugaauuccgACuCGCCg -3' miRNA: 3'- uUGCCGUGCCuGCCGcAACG---------UG-GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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