miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20848 3' -57.4 NC_004689.1 + 36312 0.66 0.665555
Target:  5'- --cGCGGggaaucGCGCCUGUuuGCG-CGCGGGGa -3'
miRNA:   3'- ucaUGCC------CGUGGACG--UGUaGCGCCUC- -5'
20848 3' -57.4 NC_004689.1 + 31948 0.66 0.654884
Target:  5'- gAGaauCGGaaccGCACC-GCGCAUUGCGGAu -3'
miRNA:   3'- -UCau-GCC----CGUGGaCGUGUAGCGCCUc -5'
20848 3' -57.4 NC_004689.1 + 64833 0.67 0.590761
Target:  5'- --cACGGGUGCCUcCACAgUGCGGGa -3'
miRNA:   3'- ucaUGCCCGUGGAcGUGUaGCGCCUc -5'
20848 3' -57.4 NC_004689.1 + 31444 0.72 0.311498
Target:  5'- uGUACGGGCaACCcgGCACuccugagaauGUCGgGGAGc -3'
miRNA:   3'- uCAUGCCCG-UGGa-CGUG----------UAGCgCCUC- -5'
20848 3' -57.4 NC_004689.1 + 8198 0.73 0.269079
Target:  5'- uGUGCGGGUACCgGUGacugCGCGGGGa -3'
miRNA:   3'- uCAUGCCCGUGGaCGUgua-GCGCCUC- -5'
20848 3' -57.4 NC_004689.1 + 10614 0.74 0.256012
Target:  5'- cGGUcuGCGGaGCGCCUGCcggagACGUUGCGGGc -3'
miRNA:   3'- -UCA--UGCC-CGUGGACG-----UGUAGCGCCUc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.