Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20848 | 3' | -57.4 | NC_004689.1 | + | 36312 | 0.66 | 0.665555 |
Target: 5'- --cGCGGggaaucGCGCCUGUuuGCG-CGCGGGGa -3' miRNA: 3'- ucaUGCC------CGUGGACG--UGUaGCGCCUC- -5' |
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20848 | 3' | -57.4 | NC_004689.1 | + | 31948 | 0.66 | 0.654884 |
Target: 5'- gAGaauCGGaaccGCACC-GCGCAUUGCGGAu -3' miRNA: 3'- -UCau-GCC----CGUGGaCGUGUAGCGCCUc -5' |
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20848 | 3' | -57.4 | NC_004689.1 | + | 64833 | 0.67 | 0.590761 |
Target: 5'- --cACGGGUGCCUcCACAgUGCGGGa -3' miRNA: 3'- ucaUGCCCGUGGAcGUGUaGCGCCUc -5' |
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20848 | 3' | -57.4 | NC_004689.1 | + | 31444 | 0.72 | 0.311498 |
Target: 5'- uGUACGGGCaACCcgGCACuccugagaauGUCGgGGAGc -3' miRNA: 3'- uCAUGCCCG-UGGa-CGUG----------UAGCgCCUC- -5' |
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20848 | 3' | -57.4 | NC_004689.1 | + | 8198 | 0.73 | 0.269079 |
Target: 5'- uGUGCGGGUACCgGUGacugCGCGGGGa -3' miRNA: 3'- uCAUGCCCGUGGaCGUgua-GCGCCUC- -5' |
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20848 | 3' | -57.4 | NC_004689.1 | + | 10614 | 0.74 | 0.256012 |
Target: 5'- cGGUcuGCGGaGCGCCUGCcggagACGUUGCGGGc -3' miRNA: 3'- -UCA--UGCC-CGUGGACG-----UGUAGCGCCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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