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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20849 | 5' | -55.9 | NC_004689.1 | + | 16294 | 0.66 | 0.74329 |
Target: 5'- --gGCagaAGCGGUGCgCCGUgGGUUGGCu -3' miRNA: 3'- ugaUGg--UCGCCAUG-GGCGaCUAGCUG- -5' |
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20849 | 5' | -55.9 | NC_004689.1 | + | 41774 | 0.66 | 0.732973 |
Target: 5'- cGCUugCAGcCGGUugCCGCccagUGccUUGACg -3' miRNA: 3'- -UGAugGUC-GCCAugGGCG----ACu-AGCUG- -5' |
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20849 | 5' | -55.9 | NC_004689.1 | + | 29659 | 0.67 | 0.65214 |
Target: 5'- gGC-ACCAGCGGgagccaGCaauucggccuuaccuCCGCUGAUCGcACg -3' miRNA: 3'- -UGaUGGUCGCCa-----UG---------------GGCGACUAGC-UG- -5' |
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20849 | 5' | -55.9 | NC_004689.1 | + | 56306 | 0.68 | 0.615375 |
Target: 5'- cUUGCC-GUGGUACCCGgUGuuguucgCGACa -3' miRNA: 3'- uGAUGGuCGCCAUGGGCgACua-----GCUG- -5' |
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20849 | 5' | -55.9 | NC_004689.1 | + | 15734 | 0.71 | 0.459192 |
Target: 5'- cCUGCCAGCGGUGCCUuggGCUuGcucucugccAUCGAUc -3' miRNA: 3'- uGAUGGUCGCCAUGGG---CGA-C---------UAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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