miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20849 5' -55.9 NC_004689.1 + 16294 0.66 0.74329
Target:  5'- --gGCagaAGCGGUGCgCCGUgGGUUGGCu -3'
miRNA:   3'- ugaUGg--UCGCCAUG-GGCGaCUAGCUG- -5'
20849 5' -55.9 NC_004689.1 + 41774 0.66 0.732973
Target:  5'- cGCUugCAGcCGGUugCCGCccagUGccUUGACg -3'
miRNA:   3'- -UGAugGUC-GCCAugGGCG----ACu-AGCUG- -5'
20849 5' -55.9 NC_004689.1 + 29659 0.67 0.65214
Target:  5'- gGC-ACCAGCGGgagccaGCaauucggccuuaccuCCGCUGAUCGcACg -3'
miRNA:   3'- -UGaUGGUCGCCa-----UG---------------GGCGACUAGC-UG- -5'
20849 5' -55.9 NC_004689.1 + 56306 0.68 0.615375
Target:  5'- cUUGCC-GUGGUACCCGgUGuuguucgCGACa -3'
miRNA:   3'- uGAUGGuCGCCAUGGGCgACua-----GCUG- -5'
20849 5' -55.9 NC_004689.1 + 15734 0.71 0.459192
Target:  5'- cCUGCCAGCGGUGCCUuggGCUuGcucucugccAUCGAUc -3'
miRNA:   3'- uGAUGGUCGCCAUGGG---CGA-C---------UAGCUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.