Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20851 | 3' | -57 | NC_004689.1 | + | 11378 | 0.66 | 0.693189 |
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Target: 5'- -cACGGCuGcGGCCaGUgGAGCgCGGAAAu -3' miRNA: 3'- guUGCCG-CaUCGG-CAgCUCG-GCCUUU- -5' |
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| 20851 | 3' | -57 | NC_004689.1 | + | 13911 | 0.67 | 0.628898 |
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Target: 5'- aAGCGGCGU---CGUCGcccucaccggaAGCCGGAGAc -3' miRNA: 3'- gUUGCCGCAucgGCAGC-----------UCGGCCUUU- -5' |
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| 20851 | 3' | -57 | NC_004689.1 | + | 18458 | 0.67 | 0.606313 |
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Target: 5'- aAACGGUGUuGCagGUCGAGCacgcuauCGGAGAa -3' miRNA: 3'- gUUGCCGCAuCGg-CAGCUCG-------GCCUUU- -5' |
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| 20851 | 3' | -57 | NC_004689.1 | + | 10535 | 0.67 | 0.585958 |
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Target: 5'- ---aGGgGUGGCCGUU--GCCGGggGa -3' miRNA: 3'- guugCCgCAUCGGCAGcuCGGCCuuU- -5' |
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| 20851 | 3' | -57 | NC_004689.1 | + | 11130 | 0.68 | 0.554109 |
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Target: 5'- --uCGGgGuUGGCCGgggGAGCCGGAGg -3' miRNA: 3'- guuGCCgC-AUCGGCag-CUCGGCCUUu -5' |
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| 20851 | 3' | -57 | NC_004689.1 | + | 48730 | 0.71 | 0.396932 |
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Target: 5'- --cCGGCGUGuuguuGaaGUCGGGCCGGAGGu -3' miRNA: 3'- guuGCCGCAU-----CggCAGCUCGGCCUUU- -5' |
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| 20851 | 3' | -57 | NC_004689.1 | + | 58750 | 0.79 | 0.126754 |
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Target: 5'- --uUGGCGUgAGCCGagUCGAGCCGGAAc -3' miRNA: 3'- guuGCCGCA-UCGGC--AGCUCGGCCUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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