Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20852 | 3' | -50.8 | NC_004689.1 | + | 13103 | 0.68 | 0.886555 |
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Target: 5'- cGUCCCAGAUuacaccaGGAAGCCGccgcCCUuGCGGa -3' miRNA: 3'- -CAGGGUUUG-------CUUUUGGUa---GGAcCGCC- -5' |
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| 20852 | 3' | -50.8 | NC_004689.1 | + | 11093 | 0.68 | 0.879737 |
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Target: 5'- --gCCGAcCGGGAGCCG--CUGGCGGg -3' miRNA: 3'- cagGGUUuGCUUUUGGUagGACCGCC- -5' |
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| 20852 | 3' | -50.8 | NC_004689.1 | + | 29732 | 0.69 | 0.871915 |
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Target: 5'- cGUUCCAAACGAAGucACCAggaCUG-CGGc -3' miRNA: 3'- -CAGGGUUUGCUUU--UGGUag-GACcGCC- -5' |
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| 20852 | 3' | -50.8 | NC_004689.1 | + | 64422 | 0.69 | 0.855518 |
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Target: 5'- aGUUCCAGucacauuCGAAAACgGUUgUGGUGGa -3' miRNA: 3'- -CAGGGUUu------GCUUUUGgUAGgACCGCC- -5' |
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| 20852 | 3' | -50.8 | NC_004689.1 | + | 63253 | 0.69 | 0.838169 |
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Target: 5'- aGUCCCAGcGCGcuacuGCCcucGUCCugUGGCGGg -3' miRNA: 3'- -CAGGGUU-UGCuuu--UGG---UAGG--ACCGCC- -5' |
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| 20852 | 3' | -50.8 | NC_004689.1 | + | 13695 | 0.7 | 0.829159 |
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Target: 5'- cGUCaCCAGACGAAuagg--UCUGGCGGu -3' miRNA: 3'- -CAG-GGUUUGCUUuugguaGGACCGCC- -5' |
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| 20852 | 3' | -50.8 | NC_004689.1 | + | 59134 | 0.73 | 0.641558 |
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Target: 5'- --aCCGGACGGGcAACCGUCCgUGuGCGGa -3' miRNA: 3'- cagGGUUUGCUU-UUGGUAGG-AC-CGCC- -5' |
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| 20852 | 3' | -50.8 | NC_004689.1 | + | 10098 | 0.74 | 0.619341 |
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Target: 5'- cGUCCCAAACGcaacgccgcauAGGCCGUCCcaccgGGCGu -3' miRNA: 3'- -CAGGGUUUGCu----------UUUGGUAGGa----CCGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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