Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2086 | 5' | -53 | NC_001348.1 | + | 120700 | 0.66 | 0.937444 |
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Target: 5'- uUUGGAGuuaauGGAccuGUUGGacgcGGCCGCGGCGGCc -3' miRNA: 3'- -AACCUU-----UCU---CGACU----CUGGCGUCGUUG- -5' |
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| 2086 | 5' | -53 | NC_001348.1 | + | 109092 | 0.66 | 0.937444 |
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Target: 5'- uUUGGAGuuaauGGAccuGUUGGacgcGGCCGCGGCGGCc -3' miRNA: 3'- -AACCUU-----UCU---CGACU----CUGGCGUCGUUG- -5' |
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| 2086 | 5' | -53 | NC_001348.1 | + | 123969 | 0.66 | 0.914188 |
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Target: 5'- -gGGAcGGAGaccgGGGGCCGCcgggccggaggccGGCGACg -3' miRNA: 3'- aaCCUuUCUCga--CUCUGGCG-------------UCGUUG- -5' |
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| 2086 | 5' | -53 | NC_001348.1 | + | 105823 | 0.66 | 0.914188 |
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Target: 5'- -gGGAcGGAGaccgGGGGCCGCcgggccggaggccGGCGACg -3' miRNA: 3'- aaCCUuUCUCga--CUCUGGCG-------------UCGUUG- -5' |
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| 2086 | 5' | -53 | NC_001348.1 | + | 122117 | 0.68 | 0.848626 |
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Target: 5'- cUGGGAGGA--UGAGGCUGUgagAGCGGCg -3' miRNA: 3'- aACCUUUCUcgACUCUGGCG---UCGUUG- -5' |
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| 2086 | 5' | -53 | NC_001348.1 | + | 107675 | 0.68 | 0.848626 |
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Target: 5'- cUGGGAGGA--UGAGGCUGUgagAGCGGCg -3' miRNA: 3'- aACCUUUCUcgACUCUGGCG---UCGUUG- -5' |
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| 2086 | 5' | -53 | NC_001348.1 | + | 90611 | 0.71 | 0.713103 |
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Target: 5'- aUGGggGGAGUguuu-CUGCGGCAACg -3' miRNA: 3'- aACCuuUCUCGacucuGGCGUCGUUG- -5' |
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| 2086 | 5' | -53 | NC_001348.1 | + | 23645 | 0.71 | 0.691802 |
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Target: 5'- aUUGGAcuGGGCcGAGAaugguuguacUCGCGGCAACg -3' miRNA: 3'- -AACCUuuCUCGaCUCU----------GGCGUCGUUG- -5' |
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| 2086 | 5' | -53 | NC_001348.1 | + | 66487 | 1.07 | 0.004169 |
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Target: 5'- uUUGGAAAGAGCUGAGACCGCAGCAACu -3' miRNA: 3'- -AACCUUUCUCGACUCUGGCGUCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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