miRNA: 3'- gCC-AUACCGGUUAa--GCUAUGAc-CCGG- -5'
miRNA: 3'- -GCCa-UACCGGUua-----AGCUAUGa---CCCGG- -5'
miRNA: 3'- -GCCa-UACCGGUua-----AGCUAUGa---CCCGG- -5'
miRNA: 3'- -GCCAUACCGGUUAAGCUAUGACCCGG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2087 | 5' | -51.3 | NC_001348.1 | + | 91452 | 0.69 | 0.921167 |
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Target: 5'- gGGuUGUGGCCAGUaacCGGUAUUcaGGCCu -3' miRNA: 3'- gCC-AUACCGGUUAa--GCUAUGAc-CCGG- -5' |
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| 2087 | 5' | -51.3 | NC_001348.1 | + | 122445 | 0.73 | 0.788134 |
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Target: 5'- gCGGccGUGGCUAugagccgUCGAUACgaccGGGCCc -3' miRNA: 3'- -GCCa-UACCGGUua-----AGCUAUGa---CCCGG- -5' |
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| 2087 | 5' | -51.3 | NC_001348.1 | + | 107347 | 0.73 | 0.788134 |
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Target: 5'- gCGGccGUGGCUAugagccgUCGAUACgaccGGGCCc -3' miRNA: 3'- -GCCa-UACCGGUua-----AGCUAUGa---CCCGG- -5' |
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| 2087 | 5' | -51.3 | NC_001348.1 | + | 66692 | 1.14 | 0.003513 |
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Target: 5'- uCGGUAUGGCCAAUUCGAUACUGGGCCa -3' miRNA: 3'- -GCCAUACCGGUUAAGCUAUGACCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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