Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20870 | 3' | -56.3 | NC_004689.1 | + | 52358 | 0.66 | 0.740051 |
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Target: 5'- uUCGgcuugCCGGUGuuGUCGAUCuuGCCu -3' miRNA: 3'- -AGUaa---GGUCACgcCAGUUGGggCGGu -5' |
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| 20870 | 3' | -56.3 | NC_004689.1 | + | 39473 | 0.67 | 0.655463 |
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Target: 5'- aUCAUggCCGGUGCcuGUCggUgCUGCCAg -3' miRNA: 3'- -AGUAa-GGUCACGc-CAGuuGgGGCGGU- -5' |
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| 20870 | 3' | -56.3 | NC_004689.1 | + | 22326 | 0.68 | 0.624195 |
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Target: 5'- cCAaUCCAucugcggccuugccUGCGGUCGgguagccgcugacaACCCCGCCAg -3' miRNA: 3'- aGUaAGGUc-------------ACGCCAGU--------------UGGGGCGGU- -5' |
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| 20870 | 3' | -56.3 | NC_004689.1 | + | 16669 | 0.68 | 0.612338 |
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Target: 5'- gCGUUCCAcgcuUGCGGaccUCGACCUCGCg- -3' miRNA: 3'- aGUAAGGUc---ACGCC---AGUUGGGGCGgu -5' |
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| 20870 | 3' | -56.3 | NC_004689.1 | + | 10651 | 0.68 | 0.612338 |
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Target: 5'- uUCAcgUCCAcacUGG-CAGCCCCGCCAg -3' miRNA: 3'- -AGUa-AGGUcacGCCaGUUGGGGCGGU- -5' |
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| 20870 | 3' | -56.3 | NC_004689.1 | + | 31526 | 0.69 | 0.548302 |
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Target: 5'- gUCAcUUCCGGUGUGGUgaucaaCGAUCCC-CCAc -3' miRNA: 3'- -AGU-AAGGUCACGCCA------GUUGGGGcGGU- -5' |
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| 20870 | 3' | -56.3 | NC_004689.1 | + | 47885 | 0.74 | 0.303108 |
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Target: 5'- cCAUUCaacagcgauggacuGUGCGGUCAA-CCCGCCAa -3' miRNA: 3'- aGUAAGgu------------CACGCCAGUUgGGGCGGU- -5' |
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| 20870 | 3' | -56.3 | NC_004689.1 | + | 11958 | 0.74 | 0.267259 |
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Target: 5'- gCGUaCCGGUGCGGU--ACCgCCGCCGa -3' miRNA: 3'- aGUAaGGUCACGCCAguUGG-GGCGGU- -5' |
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| 20870 | 3' | -56.3 | NC_004689.1 | + | 53216 | 0.75 | 0.254165 |
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Target: 5'- cUCGgcagCCGGUucgucaGCGGaggCAGCCCCGCCAg -3' miRNA: 3'- -AGUaa--GGUCA------CGCCa--GUUGGGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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