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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20870 | 5' | -55.8 | NC_004689.1 | + | 40224 | 0.66 | 0.726924 |
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Target: 5'- cACCGugGAGGaUCgugcaAUCGGCgCUGGCa- -3' miRNA: 3'- -UGGUugCUUC-AG-----UGGCCGaGACCGau -5' |
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| 20870 | 5' | -55.8 | NC_004689.1 | + | 58337 | 0.67 | 0.66243 |
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Target: 5'- cGCCAugGGAGaggcaACCGGCUUcGGUg- -3' miRNA: 3'- -UGGUugCUUCag---UGGCCGAGaCCGau -5' |
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| 20870 | 5' | -55.8 | NC_004689.1 | + | 25746 | 0.68 | 0.584954 |
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Target: 5'- uGCCAGCcccaugaGAAcGUCuCCGGCgUCUGGCa- -3' miRNA: 3'- -UGGUUG-------CUU-CAGuGGCCG-AGACCGau -5' |
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| 20870 | 5' | -55.8 | NC_004689.1 | + | 13503 | 0.68 | 0.564421 |
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Target: 5'- cGCCGGgGGAGUUGaccggaauuuCCGGCUCgacGGCUAc -3' miRNA: 3'- -UGGUUgCUUCAGU----------GGCCGAGa--CCGAU- -5' |
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| 20870 | 5' | -55.8 | NC_004689.1 | + | 52490 | 0.69 | 0.553689 |
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Target: 5'- uCCGGCucgGGAGUCGgcuCCGGCUCcGGCUu -3' miRNA: 3'- uGGUUG---CUUCAGU---GGCCGAGaCCGAu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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