Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20874 | 5' | -56.6 | NC_004689.1 | + | 7548 | 0.68 | 0.618657 |
Target: 5'- -aGUGCGgcacGCuGGCGUGCGGUcaUCUUCc -3' miRNA: 3'- agCGCGCa---UGuCCGCACGCUA--AGGGG- -5' |
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20874 | 5' | -56.6 | NC_004689.1 | + | 37284 | 0.69 | 0.555372 |
Target: 5'- -gGCGCGgucuucGCGGGCuuggGUGCGG-UCUCCg -3' miRNA: 3'- agCGCGCa-----UGUCCG----CACGCUaAGGGG- -5' |
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20874 | 5' | -56.6 | NC_004689.1 | + | 36394 | 0.7 | 0.514219 |
Target: 5'- aUCGCGCuUGCAGGCGcagggaauCGcgUCCCa -3' miRNA: 3'- -AGCGCGcAUGUCCGCac------GCuaAGGGg -5' |
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20874 | 5' | -56.6 | NC_004689.1 | + | 21721 | 0.7 | 0.50412 |
Target: 5'- gUUGCGgG-GCAGGCGUGUugggagccGGUgCCCCa -3' miRNA: 3'- -AGCGCgCaUGUCCGCACG--------CUAaGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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