Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20880 | 3' | -51.1 | NC_004689.1 | + | 46502 | 0.66 | 0.950579 |
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Target: 5'- --gGAuUCGAGCGU---AAUCACGGCGu -3' miRNA: 3'- agaCU-AGCUCGCGcguUUAGUGCUGC- -5' |
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| 20880 | 3' | -51.1 | NC_004689.1 | + | 63570 | 0.66 | 0.941081 |
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Target: 5'- cUUGAUCGAGaagccaGCGCAuguuGUCGuaugUGACGu -3' miRNA: 3'- aGACUAGCUCg-----CGCGUu---UAGU----GCUGC- -5' |
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| 20880 | 3' | -51.1 | NC_004689.1 | + | 11916 | 0.67 | 0.918752 |
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Target: 5'- --cGcgCGAGaaaGCGCGGAUCAUG-CGg -3' miRNA: 3'- agaCuaGCUCg--CGCGUUUAGUGCuGC- -5' |
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| 20880 | 3' | -51.1 | NC_004689.1 | + | 15383 | 0.67 | 0.912471 |
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Target: 5'- cCUGGUCGAGCcaaCGCG--UgACGAUGa -3' miRNA: 3'- aGACUAGCUCGc--GCGUuuAgUGCUGC- -5' |
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| 20880 | 3' | -51.1 | NC_004689.1 | + | 1866 | 0.67 | 0.905912 |
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Target: 5'- cUUGGUCGccuugcccuuGGCGCGCuGAUC-CGAUGc -3' miRNA: 3'- aGACUAGC----------UCGCGCGuUUAGuGCUGC- -5' |
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| 20880 | 3' | -51.1 | NC_004689.1 | + | 24798 | 0.68 | 0.884601 |
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Target: 5'- cCUGAUUGAGCaccacgGCGUuuucGAUCACGuCGu -3' miRNA: 3'- aGACUAGCUCG------CGCGu---UUAGUGCuGC- -5' |
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| 20880 | 3' | -51.1 | NC_004689.1 | + | 66808 | 0.68 | 0.876967 |
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Target: 5'- aCUGccccaccauUUGAGCGUGCGGAUCAUGGu- -3' miRNA: 3'- aGACu--------AGCUCGCGCGUUUAGUGCUgc -5' |
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| 20880 | 3' | -51.1 | NC_004689.1 | + | 38095 | 0.69 | 0.860938 |
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Target: 5'- gUCUGAUCGu-CGuCGUAGAgCACGAUGg -3' miRNA: 3'- -AGACUAGCucGC-GCGUUUaGUGCUGC- -5' |
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| 20880 | 3' | -51.1 | NC_004689.1 | + | 39367 | 0.71 | 0.725876 |
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Target: 5'- gCUGGUCGGcaaucaacGCGCGCAAcUCGCGcaGCa -3' miRNA: 3'- aGACUAGCU--------CGCGCGUUuAGUGC--UGc -5' |
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| 20880 | 3' | -51.1 | NC_004689.1 | + | 47237 | 0.73 | 0.616209 |
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Target: 5'- gUCUGAcucggCGGGgGCagucGCGGAUCGCGGCGu -3' miRNA: 3'- -AGACUa----GCUCgCG----CGUUUAGUGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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