Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20882 | 3' | -53.2 | NC_004689.1 | + | 12017 | 0.66 | 0.857584 |
Target: 5'- -aGGUCCgugCCaucgGCGGUGGCGGugacaaGAUCGc -3' miRNA: 3'- ugCUAGGa--GG----UGCCACCGCU------UUAGUc -5' |
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20882 | 3' | -53.2 | NC_004689.1 | + | 58590 | 0.67 | 0.838856 |
Target: 5'- cGCGGUUCUCUGCGGUGcaaauccaguaGAAAUCAu -3' miRNA: 3'- -UGCUAGGAGGUGCCACcg---------CUUUAGUc -5' |
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20882 | 3' | -53.2 | NC_004689.1 | + | 40693 | 0.69 | 0.712661 |
Target: 5'- uGCGcgCCuugUCCACGGcGGCGAGcauuUCGGc -3' miRNA: 3'- -UGCuaGG---AGGUGCCaCCGCUUu---AGUC- -5' |
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20882 | 3' | -53.2 | NC_004689.1 | + | 10974 | 0.7 | 0.647646 |
Target: 5'- -aGAcCCUCgACGGUGGCGAuuugcuuGUCGa -3' miRNA: 3'- ugCUaGGAGgUGCCACCGCUu------UAGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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