Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20885 | 3' | -55.5 | NC_004689.1 | + | 27166 | 0.66 | 0.771737 |
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Target: 5'- -gCACCGUGAuucggccuuguUCGUCUGCGAagccgAACAUu -3' miRNA: 3'- uaGUGGCGCU-----------GGCAGACGCUg----UUGUG- -5' |
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| 20885 | 3' | -55.5 | NC_004689.1 | + | 56415 | 0.66 | 0.761703 |
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Target: 5'- aAUCGuuGCGcuuAUCGUCUGUuuCGACGCg -3' miRNA: 3'- -UAGUggCGC---UGGCAGACGcuGUUGUG- -5' |
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| 20885 | 3' | -55.5 | NC_004689.1 | + | 42362 | 0.66 | 0.758667 |
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Target: 5'- cUCGCCGUcucggccaccagggGACCGUaCUGCGGgcAUACg -3' miRNA: 3'- uAGUGGCG--------------CUGGCA-GACGCUguUGUG- -5' |
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| 20885 | 3' | -55.5 | NC_004689.1 | + | 5067 | 0.67 | 0.688453 |
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Target: 5'- cGUCAUUGCGGacaCGgcgaaaugUGCGACGACGCg -3' miRNA: 3'- -UAGUGGCGCUg--GCag------ACGCUGUUGUG- -5' |
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| 20885 | 3' | -55.5 | NC_004689.1 | + | 8642 | 0.67 | 0.682001 |
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Target: 5'- -cCACCGUgccagcguugaaaccGGCUGUCUGCGA--ACGCu -3' miRNA: 3'- uaGUGGCG---------------CUGGCAGACGCUguUGUG- -5' |
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| 20885 | 3' | -55.5 | NC_004689.1 | + | 22938 | 0.67 | 0.677691 |
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Target: 5'- -gCACCGgGACgGgCUGCGACGA-ACg -3' miRNA: 3'- uaGUGGCgCUGgCaGACGCUGUUgUG- -5' |
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| 20885 | 3' | -55.5 | NC_004689.1 | + | 11772 | 0.68 | 0.634323 |
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Target: 5'- uUCGCCGCGAUuaCG-CUGCGGCGuccuucucguuCACg -3' miRNA: 3'- uAGUGGCGCUG--GCaGACGCUGUu----------GUG- -5' |
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| 20885 | 3' | -55.5 | NC_004689.1 | + | 19817 | 0.68 | 0.634323 |
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Target: 5'- uUCGCCGCuuCgGUCUGCGA--ACAUg -3' miRNA: 3'- uAGUGGCGcuGgCAGACGCUguUGUG- -5' |
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| 20885 | 3' | -55.5 | NC_004689.1 | + | 23192 | 0.7 | 0.495951 |
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Target: 5'- aGUCACCGgGACCGcCUGUgccacgGACGAaucCACg -3' miRNA: 3'- -UAGUGGCgCUGGCaGACG------CUGUU---GUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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