Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20887 | 3' | -58.5 | NC_004689.1 | + | 51518 | 0.66 | 0.640296 |
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Target: 5'- aGACGGucuacugugcgguAGCCGaCUGCGcuuccGGUGGuuGc -3' miRNA: 3'- gCUGCU-------------UCGGC-GACGCuu---CCACCggC- -5' |
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| 20887 | 3' | -58.5 | NC_004689.1 | + | 26532 | 0.66 | 0.630804 |
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Target: 5'- uGACGAAuGCaCGCgacgaGCGAAGucGUGcGCCGu -3' miRNA: 3'- gCUGCUU-CG-GCGa----CGCUUC--CAC-CGGC- -5' |
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| 20887 | 3' | -58.5 | NC_004689.1 | + | 60942 | 0.67 | 0.578252 |
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Target: 5'- cCGGCGcuGgUGCUGCccAGGUGGCgCGu -3' miRNA: 3'- -GCUGCuuCgGCGACGcuUCCACCG-GC- -5' |
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| 20887 | 3' | -58.5 | NC_004689.1 | + | 40151 | 0.67 | 0.557463 |
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Target: 5'- gCGGCGcucGCaCGCUGCGAcGGUgacuccGGCUGg -3' miRNA: 3'- -GCUGCuu-CG-GCGACGCUuCCA------CCGGC- -5' |
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| 20887 | 3' | -58.5 | NC_004689.1 | + | 56748 | 0.67 | 0.547148 |
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Target: 5'- -uGCGcaucAAGCCGaUUGCGcccauGGUGGCCGa -3' miRNA: 3'- gcUGC----UUCGGC-GACGCuu---CCACCGGC- -5' |
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| 20887 | 3' | -58.5 | NC_004689.1 | + | 47811 | 0.68 | 0.486746 |
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Target: 5'- aCGGCGAAGUCGCUGU--AGG-GGaCGa -3' miRNA: 3'- -GCUGCUUCGGCGACGcuUCCaCCgGC- -5' |
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| 20887 | 3' | -58.5 | NC_004689.1 | + | 10567 | 0.69 | 0.471165 |
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Target: 5'- gGAUuGGGCUGCUGCuguucgggauuggccGGagGGGUGGCCGu -3' miRNA: 3'- gCUGcUUCGGCGACG---------------CU--UCCACCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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