Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20888 | 5' | -55.6 | NC_004689.1 | + | 60267 | 0.67 | 0.745555 |
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Target: 5'- --gUCCCGGCaC-CUGUCGUUg-CAGAu -3' miRNA: 3'- cgaAGGGCCG-GuGGCAGCAAagGUCU- -5' |
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| 20888 | 5' | -55.6 | NC_004689.1 | + | 30544 | 0.67 | 0.735355 |
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Target: 5'- --gUUCCGGUCGCCca-GUUUCCAGc -3' miRNA: 3'- cgaAGGGCCGGUGGcagCAAAGGUCu -5' |
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| 20888 | 5' | -55.6 | NC_004689.1 | + | 23991 | 0.68 | 0.660723 |
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Target: 5'- uGCUUCCUGGCCcaaaucgaucaugGCguuUGUCGUUUCgAGc -3' miRNA: 3'- -CGAAGGGCCGG-------------UG---GCAGCAAAGgUCu -5' |
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| 20888 | 5' | -55.6 | NC_004689.1 | + | 30861 | 0.7 | 0.544707 |
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Target: 5'- cGUUaCCaccaGcGCCACCGgcucCGUUUCCAGAa -3' miRNA: 3'- -CGAaGGg---C-CGGUGGCa---GCAAAGGUCU- -5' |
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| 20888 | 5' | -55.6 | NC_004689.1 | + | 31662 | 0.72 | 0.43507 |
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Target: 5'- --gUCCCGGCCACgaacCGUUUCCGGc -3' miRNA: 3'- cgaAGGGCCGGUGgca-GCAAAGGUCu -5' |
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| 20888 | 5' | -55.6 | NC_004689.1 | + | 42010 | 0.77 | 0.234873 |
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Target: 5'- uGCaUUCCCGGCCACCaUCGUUguaUUCAGu -3' miRNA: 3'- -CG-AAGGGCCGGUGGcAGCAA---AGGUCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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