Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20890 | 5' | -58.9 | NC_004689.1 | + | 31452 | 0.67 | 0.500958 |
Target: 5'- cGGCCaCCUguACGg--GCaACCCGGCACUc -3' miRNA: 3'- cCUGG-GGA--UGCgaaCG-UGGGCCGUGA- -5' |
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20890 | 5' | -58.9 | NC_004689.1 | + | 40354 | 0.69 | 0.415319 |
Target: 5'- cGGCUCCgACGCUa-CACCCGGCGuCUg -3' miRNA: 3'- cCUGGGGaUGCGAacGUGGGCCGU-GA- -5' |
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20890 | 5' | -58.9 | NC_004689.1 | + | 54430 | 0.69 | 0.415319 |
Target: 5'- -aACCCUUGgGCUUGUACCuCGGguCa -3' miRNA: 3'- ccUGGGGAUgCGAACGUGG-GCCguGa -5' |
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20890 | 5' | -58.9 | NC_004689.1 | + | 7504 | 0.7 | 0.380239 |
Target: 5'- cGGCgCUUACGCUUGCGCCCacGGaACUu -3' miRNA: 3'- cCUGgGGAUGCGAACGUGGG--CCgUGA- -5' |
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20890 | 5' | -58.9 | NC_004689.1 | + | 2361 | 0.7 | 0.363458 |
Target: 5'- aGACCCCgUGCGa-UGUaACCCGGCAUc -3' miRNA: 3'- cCUGGGG-AUGCgaACG-UGGGCCGUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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