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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20893 | 5' | -58 | NC_004689.1 | + | 44875 | 0.66 | 0.65177 |
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Target: 5'- uGCGC-GGAUcGGGccuGCCGGUCACCgCu -3' miRNA: 3'- gUGCGuUCUA-CUC---CGGCCAGUGGgGu -5' |
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| 20893 | 5' | -58 | NC_004689.1 | + | 58980 | 0.66 | 0.630432 |
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Target: 5'- gAgGCcGGAguaaaGAGGuCCGGUCccuGCCCCGg -3' miRNA: 3'- gUgCGuUCUa----CUCC-GGCCAG---UGGGGU- -5' |
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| 20893 | 5' | -58 | NC_004689.1 | + | 36907 | 0.66 | 0.602711 |
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Target: 5'- -uCGguGGAgcGGGCCucgcuggccugccccGGUCGCCCCAc -3' miRNA: 3'- guGCguUCUacUCCGG---------------CCAGUGGGGU- -5' |
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| 20893 | 5' | -58 | NC_004689.1 | + | 41008 | 0.66 | 0.598457 |
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Target: 5'- aGCGCGuccuGAUcGAguuGGCCGGUgGCCCa- -3' miRNA: 3'- gUGCGUu---CUA-CU---CCGGCCAgUGGGgu -5' |
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| 20893 | 5' | -58 | NC_004689.1 | + | 62401 | 0.67 | 0.556234 |
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Target: 5'- aACGUAGGGaggaaUGAGGCCGaacuUCcgACCCCAc -3' miRNA: 3'- gUGCGUUCU-----ACUCCGGCc---AG--UGGGGU- -5' |
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| 20893 | 5' | -58 | NC_004689.1 | + | 38503 | 0.67 | 0.535436 |
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Target: 5'- cCACGUucauGAcGAGGuuGccGUCGCCCCAc -3' miRNA: 3'- -GUGCGuu--CUaCUCCggC--CAGUGGGGU- -5' |
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| 20893 | 5' | -58 | NC_004689.1 | + | 21716 | 0.67 | 0.535436 |
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Target: 5'- gGgGCAGGcguguUGGGaGCCGGU-GCCCCAg -3' miRNA: 3'- gUgCGUUCu----ACUC-CGGCCAgUGGGGU- -5' |
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| 20893 | 5' | -58 | NC_004689.1 | + | 60251 | 0.73 | 0.248642 |
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Target: 5'- -uUGC-AGAUGAGGCaCGaUCACCCCAc -3' miRNA: 3'- guGCGuUCUACUCCG-GCcAGUGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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