Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20894 | 3' | -49.4 | NC_004689.1 | + | 15420 | 0.66 | 0.983676 |
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Target: 5'- aGGUcucGCggaccCCGGuUGAUCGUUGGGUGc -3' miRNA: 3'- gCCA---UGaa---GGCU-AUUGGCAACCCAUc -5' |
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| 20894 | 3' | -49.4 | NC_004689.1 | + | 48399 | 0.66 | 0.976644 |
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Target: 5'- uGGUACUUCuCGAUGACUuuuuguUUGGcGUAu -3' miRNA: 3'- gCCAUGAAG-GCUAUUGGc-----AACC-CAUc -5' |
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| 20894 | 3' | -49.4 | NC_004689.1 | + | 29220 | 0.67 | 0.964111 |
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Target: 5'- cCGGUACgaaUCgcaGAUGGCCGgaacGGGUGa -3' miRNA: 3'- -GCCAUGa--AGg--CUAUUGGCaa--CCCAUc -5' |
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| 20894 | 3' | -49.4 | NC_004689.1 | + | 12448 | 0.69 | 0.913595 |
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Target: 5'- gCGGUGCaacgccgUCCGu--GCCGUUguagagcGGGUAGa -3' miRNA: 3'- -GCCAUGa------AGGCuauUGGCAA-------CCCAUC- -5' |
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| 20894 | 3' | -49.4 | NC_004689.1 | + | 67634 | 0.7 | 0.893791 |
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Target: 5'- aCGGUGCagcgugUCCGGUGAagcCCGUUGGcGgGGg -3' miRNA: 3'- -GCCAUGa-----AGGCUAUU---GGCAACC-CaUC- -5' |
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| 20894 | 3' | -49.4 | NC_004689.1 | + | 49122 | 0.77 | 0.50711 |
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Target: 5'- gGGUguuACUUCUGAaAACCGUUGGGgAGa -3' miRNA: 3'- gCCA---UGAAGGCUaUUGGCAACCCaUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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