Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20896 | 3' | -59.3 | NC_004689.1 | + | 31760 | 0.66 | 0.594402 |
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Target: 5'- cGCAGGCCAAUCc-GCUCGaugcGGUCGg -3' miRNA: 3'- -UGUCCGGUUGGcaCGGGCagu-CCAGC- -5' |
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| 20896 | 3' | -59.3 | NC_004689.1 | + | 27335 | 0.66 | 0.573526 |
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Target: 5'- aACAcGGCCAACUGcugcaacGCCUGUCcgAGGaUCGg -3' miRNA: 3'- -UGU-CCGGUUGGCa------CGGGCAG--UCC-AGC- -5' |
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| 20896 | 3' | -59.3 | NC_004689.1 | + | 44331 | 0.67 | 0.482491 |
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Target: 5'- gACAGGCUcaaAGCCGaUGC--GUCGGGUCa -3' miRNA: 3'- -UGUCCGG---UUGGC-ACGggCAGUCCAGc -5' |
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| 20896 | 3' | -59.3 | NC_004689.1 | + | 10079 | 0.68 | 0.444281 |
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Target: 5'- cAUAGGCCGucccACCGgGCgUGcCGGGUCGu -3' miRNA: 3'- -UGUCCGGU----UGGCaCGgGCaGUCCAGC- -5' |
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| 20896 | 3' | -59.3 | NC_004689.1 | + | 44619 | 0.69 | 0.405171 |
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Target: 5'- -uGGGCCAuguCCgGUGUCCGUUucuucauggugcagAGGUCGa -3' miRNA: 3'- ugUCCGGUu--GG-CACGGGCAG--------------UCCAGC- -5' |
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| 20896 | 3' | -59.3 | NC_004689.1 | + | 12887 | 0.7 | 0.348748 |
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Target: 5'- cGCAGGCCGACCG-GgUUGcgaAGGUCGa -3' miRNA: 3'- -UGUCCGGUUGGCaCgGGCag-UCCAGC- -5' |
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| 20896 | 3' | -59.3 | NC_004689.1 | + | 40892 | 0.71 | 0.293914 |
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Target: 5'- gUAGGUCGuACCGUcGCCguucggaucgcgcaCGUCAGGUCGa -3' miRNA: 3'- uGUCCGGU-UGGCA-CGG--------------GCAGUCCAGC- -5' |
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| 20896 | 3' | -59.3 | NC_004689.1 | + | 11873 | 0.75 | 0.166209 |
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Target: 5'- cGCuGGCCGACCGcGCacuuCCGUUGGGUCGc -3' miRNA: 3'- -UGuCCGGUUGGCaCG----GGCAGUCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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