Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20898 | 3' | -59.8 | NC_004689.1 | + | 53280 | 0.66 | 0.574545 |
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Target: 5'- --cGACCuCGC-CGCugUCGCCGUCGu -3' miRNA: 3'- ccuCUGG-GCGaGCGugAGCGGUGGCu -5' |
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| 20898 | 3' | -59.8 | NC_004689.1 | + | 24842 | 0.66 | 0.554074 |
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Target: 5'- gGGAGcguuGCCUGC-CGUACcCGCCACgGu -3' miRNA: 3'- -CCUC----UGGGCGaGCGUGaGCGGUGgCu -5' |
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| 20898 | 3' | -59.8 | NC_004689.1 | + | 20585 | 0.66 | 0.553056 |
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Target: 5'- cGGGGACCUGCUCGgC-CUUugcauugaggucgGCCAUCa- -3' miRNA: 3'- -CCUCUGGGCGAGC-GuGAG-------------CGGUGGcu -5' |
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| 20898 | 3' | -59.8 | NC_004689.1 | + | 23858 | 0.67 | 0.494176 |
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Target: 5'- uGGAGAgaaCCUgGCUCGUug-CGCCugCGAu -3' miRNA: 3'- -CCUCU---GGG-CGAGCGugaGCGGugGCU- -5' |
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| 20898 | 3' | -59.8 | NC_004689.1 | + | 48814 | 0.68 | 0.469137 |
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Target: 5'- --uGACCUGCUCGUcggucagccccuuguACuUCGCCGCCu- -3' miRNA: 3'- ccuCUGGGCGAGCG---------------UG-AGCGGUGGcu -5' |
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| 20898 | 3' | -59.8 | NC_004689.1 | + | 41140 | 0.69 | 0.392222 |
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Target: 5'- aGGAucaGCgCGCUCGuCACaccgaguUCGCCACCGGc -3' miRNA: 3'- -CCUc--UGgGCGAGC-GUG-------AGCGGUGGCU- -5' |
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| 20898 | 3' | -59.8 | NC_004689.1 | + | 37403 | 0.7 | 0.343889 |
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Target: 5'- cGGAcuacGCCCGCUugccguagaUGCGCUUGCCGCUGc -3' miRNA: 3'- -CCUc---UGGGCGA---------GCGUGAGCGGUGGCu -5' |
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| 20898 | 3' | -59.8 | NC_004689.1 | + | 42292 | 0.7 | 0.328507 |
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Target: 5'- cGGcGACCUGCUCGgGCaCGCC-CUGAu -3' miRNA: 3'- -CCuCUGGGCGAGCgUGaGCGGuGGCU- -5' |
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| 20898 | 3' | -59.8 | NC_004689.1 | + | 7959 | 0.71 | 0.321009 |
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Target: 5'- -cAGACCCGC-CaGCGCgUGCCGCUGAa -3' miRNA: 3'- ccUCUGGGCGaG-CGUGaGCGGUGGCU- -5' |
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| 20898 | 3' | -59.8 | NC_004689.1 | + | 16632 | 0.77 | 0.117427 |
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Target: 5'- aGGcAGACCgCGCUCGaugACaUCGCCACCGGu -3' miRNA: 3'- -CC-UCUGG-GCGAGCg--UG-AGCGGUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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