Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 209 | 5' | -62.7 | AC_000007.1 | + | 11034 | 0.67 | 0.216426 |
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Target: 5'- uCCGGU-GCuGCGGCAgauGCGccccccuccucaGCAGCGGCa -3' miRNA: 3'- -GGCCGcCGuCGCCGU---CGC------------CGUCGUUG- -5' |
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| 209 | 5' | -62.7 | AC_000007.1 | + | 8625 | 0.66 | 0.220421 |
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Target: 5'- -aGGgGGCAGgGGCAcgucggcgccgcgcGCGGgCAGgAGCu -3' miRNA: 3'- ggCCgCCGUCgCCGU--------------CGCC-GUCgUUG- -5' |
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| 209 | 5' | -62.7 | AC_000007.1 | + | 12068 | 0.66 | 0.24636 |
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Target: 5'- cCUGGCuGGCAcgGGCAGCGGCGaUAGa -3' miRNA: 3'- -GGCCG-CCGUcgCCGUCGCCGUcGUUg -5' |
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| 209 | 5' | -62.7 | AC_000007.1 | + | 27989 | 0.66 | 0.252744 |
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Target: 5'- aCCGGa-GCcGCgGGCAaagcacuuGUGGCGGCGGCa -3' miRNA: 3'- -GGCCgcCGuCG-CCGU--------CGCCGUCGUUG- -5' |
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| 209 | 5' | -62.7 | AC_000007.1 | + | 8462 | 0.66 | 0.255336 |
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Target: 5'- uUGGUGGCGGCGucgaugacuuGCAagaggccgcauccccGCGGC-GCGACu -3' miRNA: 3'- gGCCGCCGUCGC----------CGU---------------CGCCGuCGUUG- -5' |
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| 209 | 5' | -62.7 | AC_000007.1 | + | 13944 | 0.66 | 0.259264 |
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Target: 5'- uCUGGUguGGgAGgacgaugacuCGGCAGCGaCAGCAGCg -3' miRNA: 3'- -GGCCG--CCgUC----------GCCGUCGCcGUCGUUG- -5' |
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| 209 | 5' | -62.7 | AC_000007.1 | + | 25362 | 0.66 | 0.259264 |
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Target: 5'- cCUGGCaaaCGGCcaugGGCGuGUGGCAGCAAUg -3' miRNA: 3'- -GGCCGcc-GUCG----CCGU-CGCCGUCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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