Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20901 | 3' | -51.6 | NC_004689.1 | + | 44311 | 0.66 | 0.928564 |
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Target: 5'- gUCgGGUCAcuGGCcGUCGCUUuccuucuuauuagGGGCAUCc -3' miRNA: 3'- -GGgCUAGU--UCGaCAGCGAG-------------UUCGUAG- -5' |
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| 20901 | 3' | -51.6 | NC_004689.1 | + | 54232 | 0.66 | 0.910814 |
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Target: 5'- aCUGAUCcGGC--UCGUUCAGGCAg- -3' miRNA: 3'- gGGCUAGuUCGacAGCGAGUUCGUag -5' |
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| 20901 | 3' | -51.6 | NC_004689.1 | + | 60356 | 0.71 | 0.710988 |
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Target: 5'- gCCCGGUUgcGCguugaucGUCGCUuCGGGUAUCu -3' miRNA: 3'- -GGGCUAGuuCGa------CAGCGA-GUUCGUAG- -5' |
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| 20901 | 3' | -51.6 | NC_004689.1 | + | 22900 | 0.71 | 0.689111 |
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Target: 5'- gCCCGGUC---CUGUUGCUUGAGCAa- -3' miRNA: 3'- -GGGCUAGuucGACAGCGAGUUCGUag -5' |
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| 20901 | 3' | -51.6 | NC_004689.1 | + | 11052 | 0.72 | 0.644775 |
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Target: 5'- uUCgGAUCGGGCUGcUGCUCGggaccuuucgaaAGCAUCc -3' miRNA: 3'- -GGgCUAGUUCGACaGCGAGU------------UCGUAG- -5' |
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| 20901 | 3' | -51.6 | NC_004689.1 | + | 40542 | 0.72 | 0.633634 |
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Target: 5'- aCCGAUCAugcAGUUGUCGUgCAGGUAg- -3' miRNA: 3'- gGGCUAGU---UCGACAGCGaGUUCGUag -5' |
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| 20901 | 3' | -51.6 | NC_004689.1 | + | 27006 | 0.72 | 0.622491 |
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Target: 5'- uCCCGcugcGUCAAucgcgcGCUGUUGCUUgcGCAUCa -3' miRNA: 3'- -GGGC----UAGUU------CGACAGCGAGuuCGUAG- -5' |
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| 20901 | 3' | -51.6 | NC_004689.1 | + | 13729 | 0.73 | 0.567101 |
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Target: 5'- -aCGAgagCGAGCuUGUCGUUCaAAGCAUCc -3' miRNA: 3'- ggGCUa--GUUCG-ACAGCGAG-UUCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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